Jamalan Mostafa, Ardestani Susan Kaboudanian, Zeinali Majid, Mosaveri Nader, Mohammad Taheri Mohammad
Immunology Lab, Institute of Biochemistry and Biophysics, University of Tehran, Enghelab Ave, PO Box 13145-1384, Tehran, Iran.
Biologicals. 2011 Jan;39(1):23-8. doi: 10.1016/j.biologicals.2010.08.005. Epub 2010 Oct 20.
Bacterial lipopolysaccharide (LPS) has T-helper 1 (Th1) immunostimulatory activities but because of toxicity and pyrogenicity cannot be used as an adjuvant. Brucella abortus LPS has less toxicity and no pyrogenic properties in comparison to other bacterial LPS. In the current study, the immunostimulatory properties of B. abortus LPS were evaluated for its adjuvant activity. Tuberculin purified protein derivative (PPD) from Mycobacterium tuberculosis was extracted and after anion-exchange chromatography on Q-sepharose column, two fractions (17 and 23), which dominantly contained 30- and 70-kDa antigens, were collected for immunological studies. BALB/c mice were immunized with four different antigen preparations (BCG, PPD, 17th and 23rd PPD fractions) along with complete Freund's adjuvant or B. abortus LPS. The T-cell immune response of mice was assessed by measurement of Th1-type cytokine (IFN-γ) and Th2-type cytokines (IL-5 and IL-10) levels. Also, the humoral immunity was evaluated by measuring the specific IgG levels. Our results showed that immunization of mice with 17th PPD fraction along with B. abortus LPS can induce a Th1-type cytokine response characterized with a high IFN-γ/IL-5 ratio, while immunization with PPD or 23rd PPD fraction along with the same adjuvant resulted to a mixed Th1/Th2-type cytokine response.
细菌脂多糖（LPS）具有辅助性T细胞1（Th1）免疫刺激活性，但由于其毒性和致热原性而不能用作佐剂。与其他细菌LPS相比，流产布鲁氏菌LPS的毒性较小且无致热特性。在本研究中，评估了流产布鲁氏菌LPS的免疫刺激特性及其佐剂活性。从结核分枝杆菌中提取结核菌素纯化蛋白衍生物（PPD），并在Q-琼脂糖柱上进行阴离子交换色谱后，收集主要包含30 kDa和70 kDa抗原的两个组分（17和23）用于免疫学研究。用四种不同的抗原制剂（卡介苗、PPD、第17和第23个PPD组分）与完全弗氏佐剂或流产布鲁氏菌LPS一起免疫BALB/c小鼠。通过测量Th1型细胞因子（IFN-γ）和Th2型细胞因子（IL-5和IL-10）水平来评估小鼠的T细胞免疫反应。此外，通过测量特异性IgG水平来评估体液免疫。我们的结果表明，用第17个PPD组分与流产布鲁氏菌LPS一起免疫小鼠可诱导以高IFN-γ/IL-5比值为特征的Th1型细胞因子反应，而用PPD或第23个PPD组分与相同佐剂一起免疫则导致混合的Th1/Th2型细胞因子反应。
