Department of Dermatology, Fukuoka University School of Medicine, 7-45-1 Nanakuma, Jonan-ku, Fukuoka City 814-0180, Japan.
Biochem Biophys Res Commun. 2011 Jul 22;411(1):137-42. doi: 10.1016/j.bbrc.2011.06.115. Epub 2011 Jun 23.
We investigated the effects of ultrasound-mediated transfection (sonotransfection) of interferon β (IFN-β) gene on melanoma (C32) both in vitro and in vivo. C32 cells were sonotransfected with IFN-β in vitro. Subcutaneous C32 tumors in mice were sonicated weekly immediately after intra-tumor injection with IFN-β genes mixed with microbubbles. Successful sonotransfection with IFN-β gene in vitro was confirmed by ELISA, which resulted in C32 growth inhibition. In vivo, the growth ratio of tumors transfected with IFN-β gene was significantly lower than the other experimental groups. These results may lead to a new method of treatment against melanoma and other hard-to-treat cancers.
我们研究了超声介导转染（声转染）干扰素β（IFN-β）基因对体外和体内黑色素瘤（C32）的影响。在体外，C32 细胞用 IFN-β 进行声转染。在经微泡混合的 IFN-β 基因瘤内注射后，每周立即对小鼠的皮下 C32 肿瘤进行超声处理。ELISA 证实了 IFN-β 基因在体外的成功转染，导致 C32 生长抑制。在体内，转染 IFN-β 基因的肿瘤生长比例明显低于其他实验组。这些结果可能为治疗黑色素瘤和其他难以治疗的癌症提供一种新的方法。
