Department of Plant Virology, Institute of Plant Molecular Biology, Biology Centre of the Academy of Sciences of the Czech Republic, České Budějovice, Czech Republic.
Plasmid. 2011 Jul;66(2):122-7. doi: 10.1016/j.plasmid.2011.06.002. Epub 2011 Jun 23.
Complete sequences for two plasmids associated with two strains of "Candidatus Phytoplasma asteris" have been obtained. The plasmid named pPARG1 was found in Rehmannia glutinosa L. associated with phytoplasma classified in the 16Sr I-C subgroup. Plasmid pPABN1 was from phytoplasma associated with infected winter oilseed rape and classified in the 16Sr I-B subgroup. The plasmids pPARG1 (4371 nt) and pPABN1 (3529 nt) have high A+T content of about 75%, similar to that of phytoplasma genomes. Six and five open reading frames longer than 100 amino acids and organized on the same strand were recognized on pPARG1 and pPABN1, respectively. A segment about 1.6 kbp long sharing high sequence identity with the Onion yellows phytoplasma genome was found in pPABN1.
已获得与两种“植原体 asteris”菌株相关的两个质粒的完整序列。命名为 pPARG1 的质粒存在于与分类为 16Sr I-C 亚组的植原体相关的地黄中。质粒 pPABN1 来自与感染冬季油菜的植原体相关，分类为 16Sr I-B 亚组。质粒 pPARG1（4371nt）和 pPABN1（3529nt）具有约 75%的高 A+T 含量，与植原体基因组相似。在 pPARG1 和 pPABN1 上分别识别到六个和五个大于 100 个氨基酸的开放阅读框，并且在同一链上组织。在 pPABN1 中发现了一个约 1.6 kbp 长的与洋葱黄化植原体基因组具有高度序列同一性的片段。
