Samani N J, Vincent M, Sassard J, Henderson I W, Kaiser M A, Brammar W J, Swales J D
Department of Medicine, University of Leicester, UK.
J Hypertens. 1990 Sep;8(9):805-9. doi: 10.1097/00004872-199009000-00003.
The region of intron A of the rat renin gene containing a unique tandemly repeated sequence was analysed in the Milan and Lyon hypertensive rat strains and their controls, and in several Sprague-Dawley rats, using an oligonucleotide probe complementary to the tandemly repeated sequence and a renin complementary DNA probe. In the Milan rats, the size of the Bgl II DNA fragment encompassing the tandem repeat region was the same in the hypertensive (MHS) and normotensive (MNS) strains. In the Lyon model, a difference of 1.1 kilobase (equivalent to about 28 copies of the 38 basepair tandem repeat sequence) was observed in the size of the Bgl II fragment of the hypertensive (LH) and normotensive (LN) strains. However, the finding that the size of the fragment in the Lyon low-blood-pressure (LL) strain was the same as that in the LH strain rather than the LN strain suggests that the difference between the two latter strains is not by itself a major cause of the blood pressure difference between them in the intron A tandem region. An analysis of Sprague-Dawley rats, from which the Lyon strains are derived, showed that at least three different renin gene alleles, two with Bgl II fragments of the same size as those seen in the Lyon strains, are randomly segregating in this population.
利用与串联重复序列互补的寡核苷酸探针和肾素互补DNA探针，对大鼠肾素基因内含子A中包含独特串联重复序列的区域进行了分析，研究对象包括米兰和里昂高血压大鼠品系及其对照，以及几只斯普拉格-道利大鼠。在米兰大鼠中，包含串联重复区域的Bgl II DNA片段大小在高血压（MHS）和正常血压（MNS）品系中相同。在里昂模型中，观察到高血压（LH）和正常血压（LN）品系的Bgl II片段大小存在1.1千碱基的差异（相当于约28个38碱基对串联重复序列拷贝）。然而，里昂低血压（LL）品系中片段大小与LH品系相同而非LN品系，这一发现表明后两个品系之间的差异本身并非其内含子A串联区域血压差异的主要原因。对里昂品系来源的斯普拉格-道利大鼠的分析表明，至少有三个不同的肾素基因等位基因，其中两个的Bgl II片段大小与里昂品系中观察到的相同，在该群体中随机分离。
