University of Plovdiv Paisii Hilendarski, 4000 Plovdiv, Bulgaria.
J Invertebr Pathol. 2011 Sep;108(1):56-8. doi: 10.1016/j.jip.2011.05.019. Epub 2011 Jun 24.
Cry15Aa protein, produced by Bacillus thuringiensis serovar thompsoni HD542, in a crystal together with a 40 kDa accompanying protein, is one of a small group of non-typical, less well-studied members of the Cry family of insecticidal proteins, and may provide an alternative for the more commonly used Cry proteins in insect pest management. In this study we examined the role of the C-terminal part of Cry15Aa and of the 40 kDa protein in crystal formation in recombinant B. thuringiensis. The contribution of the 40 kDa protein and of the Cry15Aa carboxy-terminal sequence for crystal formation, crystal solubilization, and insecticidal properties was assessed. No significant differences in toxicity against Cydia pomonella, before or after in vitro solubilization of crystal-spore preparations, were found. Although the 40 kDa protein significantly contributes to in vitro solubility and in vivo crystal formation of Cry15Aa, no direct evidence for involvement of the 40 kDa protein in toxicity of Cry15Aa was found.
Cry15Aa 蛋白由苏云金芽孢杆菌亚种 thompsoni HD542 产生，与一个 40 kDa 的伴随蛋白一起存在于晶体中，是一类非典型的、研究较少的 Cry 家族杀虫蛋白的小成员之一，可能为更常用的 Cry 蛋白在害虫管理中提供了一种替代方法。在这项研究中，我们研究了 Cry15Aa 的 C 末端部分和 40 kDa 蛋白在重组苏云金芽孢杆菌中的晶体形成中的作用。评估了 40 kDa 蛋白和 Cry15Aa 羧基末端序列对晶体形成、晶体溶解和杀虫特性的贡献。在体外溶解晶体-孢子制剂前后，对苹果蠹蛾的毒性没有显著差异。尽管 40 kDa 蛋白显著促进了 Cry15Aa 的体外溶解度和体内晶体形成，但没有发现 40 kDa 蛋白直接参与 Cry15Aa 毒性的直接证据。
