Hernández-Martínez Patricia, Khorramnejad Ayda, Prentice Katterine, Andrés-Garrido Ascensión, Vera-Velasco Natalia Mara, Smagghe Guy, Escriche Baltasar
Laboratory of Biotechnological Control of Pest, ERI de Biotecnología y Biomedicina, Department of Genetics, Universitat de València, Burjassot, Spain.
Laboratory of Biological Control of Pest, Department of Plant Protection, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran.
Front Microbiol. 2020 Jul 22;11:1734. doi: 10.3389/fmicb.2020.01734. eCollection 2020.
The Cry23Aa/Cry37Aa proteins from (Bt) have been described toxic to larvae. In general, it is believed that Cry23Aa and Cry37Aa act jointly to exert the insecticidal activity, while there is no evidence of their toxicity individually. Therefore, in the present study, the contribution of each protein in the insecticidal activity toward larvae has been assessed. The results showed that both proteins were toxic for larvae when tested individually. Contrary to what was claimed previously, our results suggest that the presence of both proteins is not necessary to exert toxicity against larvae. Also, the binding behavior of Cry23Aa protein to midgut receptors of larvae has been determined. According to our results, Cry23Aa binds to brush border membrane vesicles (BBMV) specifically and independently of Cry37Aa. Due to the lack of common binding sites, Cry23Aa can be pyramided with Cry3Aa protein for better management of .
来自苏云金芽孢杆菌（Bt）的Cry23Aa/Cry37Aa蛋白已被描述为对甲虫幼虫有毒。一般来说，人们认为Cry23Aa和Cry37Aa共同作用发挥杀虫活性，而没有它们各自毒性的证据。因此，在本研究中，评估了每种蛋白对甲虫幼虫杀虫活性的贡献。结果表明，单独测试时两种蛋白对甲虫幼虫都有毒性。与之前所声称的相反，我们的结果表明，两种蛋白的存在对于对甲虫幼虫发挥毒性并非必要。此外，还确定了Cry23Aa蛋白与甲虫幼虫中肠受体的结合行为。根据我们的结果，Cry23Aa特异性地结合到甲虫刷状缘膜囊泡（BBMV）上，且独立于Cry37Aa。由于缺乏共同的结合位点，Cry23Aa可以与Cry3Aa蛋白进行基因叠加，以便更好地防治甲虫。
