Nordeen R O, Holloway B W
Department of Genetics and Developmental Biology, Monash University, Clayton, Victoria, Australia.
J Gen Microbiol. 1990 Jul;136(7):1231-9. doi: 10.1099/00221287-136-7-1231.
A conjugation system for mapping the chromosome of Pseudomonas syringae pv. syringae PS224 has been developed using the IncP-10 plasmid R91-5; pMO22, a Tn501-loaded derivative of R91-5; and pMO75, R91-5 loaded with Tn5. Nine different donor origins were identified with R91-5 and pMO22. By insertion of Tn5 into various sites of the chromosome, an additional six donor origins were available using pMO75 as the donor plasmid. In all, 36 markers were located on three linkage groups. Many donor strains were unstable and the limited availability of stable donor strains has limited the extent to which markers have been located. This instability of donor strains is in marked contrast to the highly stable donor strains found in P. putida using the same plasmids. As in P. aeruginosa and P. putida, auxotrophic markers in P. syringae do not show the clustering of related markers found in enterobacteria.
利用IncP - 10质粒R91 - 5、pMO22（R91 - 5携带Tn501的衍生物）和pMO75（R91 - 5携带Tn5）构建了一个用于绘制丁香假单胞菌丁香致病变种PS224染色体图谱的接合系统。使用R91 - 5和pMO22鉴定出9个不同的供体来源。通过将Tn5插入染色体的各个位点，以pMO75作为供体质粒又获得了另外6个供体来源。总共36个标记位于三个连锁群上。许多供体菌株不稳定，稳定供体菌株的有限可用性限制了标记定位的程度。供体菌株的这种不稳定性与在恶臭假单胞菌中使用相同质粒发现的高度稳定的供体菌株形成了鲜明对比。与铜绿假单胞菌和恶臭假单胞菌一样，丁香假单胞菌中的营养缺陷型标记并不表现出肠杆菌中相关标记的聚类现象。
