铜绿假单胞菌PAT中的染色体图谱分析
Chromosome mapping in Pseudomonas aeruginosa PAT.
作者信息
Watson J M, Holloway B W
出版信息
J Bacteriol. 1978 Mar;133(3):1113-25. doi: 10.1128/jb.133.3.1113-1125.1978.
Abstract
A linkage map of Pseudomonas aeruginosa PAT has been derived from the results of conjugation experiments using the plasmids FP2-2, R68, R91-5, and R68.45. FP2-2 and R68 each mobilize the chromosome from single, distinct transfer origins. R91-5 appears to mobilize the chromosome from two such origins, and R68.45 utilizes a number of transfer origins. R68 and R91-5 have both been shown to mobilize the chromosome with a polarity opposite to that by FP2-2. The locations of the transfer origins of these plasmids are such that it has not been possible to demonstrate chromosomal circularity by means of interrupted mating experiments. However, the available time-of-entry data combined with linkage data from plate mating experiments support the conclusion that the chromosome of P. aeruginosa is circular.
摘要
利用质粒FP2 - 2、R68、R91 - 5和R68.45进行接合实验，得出了铜绿假单胞菌PAT的连锁图谱。FP2 - 2和R68各自从单一、不同的转移起点动员染色体。R91 - 5似乎从两个这样的起点动员染色体，而R68.45利用多个转移起点。已证明R68和R91 - 5动员染色体的极性与FP2 - 2相反。这些质粒转移起点的位置使得无法通过中断杂交实验证明染色体的环状性。然而，现有的进入时间数据与平板交配实验的连锁数据相结合，支持了铜绿假单胞菌染色体是环状的这一结论。
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