技术说明：酶联免疫吸附测定法测定猪饲料中氟苯尼考和甲砜霉素的研究。
Technical note: Development of an enzyme-linked immunosorbent assay for the determination of florfenicol and thiamphenicol in swine feed.
机构信息
Key Laboratory of Chemical Safety and Health, National Institute for Nutrition and Food Safety, Chinese Center for Disease Control and Prevention, Beijing 100050, People's Republic of China.
出版信息
J Anim Sci. 2011 Nov;89(11):3612-6. doi: 10.2527/jas.2010-3403. Epub 2011 Jul 1.
One polyclonal antibody against florfenicol and thiamphenicol was produced and a competitive ELISA was developed for the detection of florfenicol and thiamphenicol in swine feed. The ELISA gave a 50% inhibiting concentration of 1.02 ng/mL for florfenicol. For swine feed fortified with 0.05 to 3.0 mg/kg, the interassay recoveries of florfenicol and thiamphenicol ranged from 86.4 to 118.6%, whereas intraassay recoveries of both drug ranged from 90.1 to 126.5% with less than 15% CV. Results obtained from HPLC-tandem mass spectrometry indicated this ELISA procedure could be used as a convenient method for rapid screening of florfenicol and thiamphenicol in swine feed.
制备了一种针对氟苯尼考和甲砜霉素的多克隆抗体，并建立了用于检测猪饲料中氟苯尼考和甲砜霉素的竞争 ELISA 方法。该 ELISA 方法对氟苯尼考的 50%抑制浓度为 1.02ng/mL。对于添加浓度为 0.05 至 3.0mg/kg 的猪饲料，氟苯尼考和甲砜霉素的回收率在 86.4%至 118.6%之间，而两种药物的日内回收率在 90.1%至 126.5%之间，变异系数小于 15%。高效液相色谱-串联质谱法的结果表明，该 ELISA 方法可作为一种快速筛选猪饲料中氟苯尼考和甲砜霉素的简便方法。
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