Key Laboratory of Chemical Safety and Health, National Institute for Nutrition and Food Safety, Chinese Center for Disease Control and Prevention, Beijing 100050, People's Republic of China.
J Anim Sci. 2011 Nov;89(11):3612-6. doi: 10.2527/jas.2010-3403. Epub 2011 Jul 1.
One polyclonal antibody against florfenicol and thiamphenicol was produced and a competitive ELISA was developed for the detection of florfenicol and thiamphenicol in swine feed. The ELISA gave a 50% inhibiting concentration of 1.02 ng/mL for florfenicol. For swine feed fortified with 0.05 to 3.0 mg/kg, the interassay recoveries of florfenicol and thiamphenicol ranged from 86.4 to 118.6%, whereas intraassay recoveries of both drug ranged from 90.1 to 126.5% with less than 15% CV. Results obtained from HPLC-tandem mass spectrometry indicated this ELISA procedure could be used as a convenient method for rapid screening of florfenicol and thiamphenicol in swine feed.
制备了一种针对氟苯尼考和甲砜霉素的多克隆抗体，并建立了用于检测猪饲料中氟苯尼考和甲砜霉素的竞争 ELISA 方法。该 ELISA 方法对氟苯尼考的 50%抑制浓度为 1.02ng/mL。对于添加浓度为 0.05 至 3.0mg/kg 的猪饲料，氟苯尼考和甲砜霉素的回收率在 86.4%至 118.6%之间，而两种药物的日内回收率在 90.1%至 126.5%之间，变异系数小于 15%。高效液相色谱-串联质谱法的结果表明，该 ELISA 方法可作为一种快速筛选猪饲料中氟苯尼考和甲砜霉素的简便方法。
