Tada Yoshiteru, Kanematsu Yasuhisa, Kanematsu Miyuki, Nuki Yoshitsugu, Liang Elena I, Wada Kosuke, Makino Hiroshi, Hashimoto Tomoki
Department of Anesthesia and Perioperative Care, Center for Cerebrovascular Research, University of California, 1001, Potrero Avenue, No. 3C-38, San Francisco, CA 94110, USA.
Acta Neurochir Suppl. 2011;111:31-5. doi: 10.1007/978-3-7091-0693-8_6.
Intracranial aneurysms can be induced by a single stereotaxic injection of elastase into the cerebrospinal fluid at the right basal cistern in hypertensive mice. This mouse model produces large aneurysm formations with an incidence of 60-80% within 3-4 weeks. Intracranial aneurysms in this model recapitulate key pathological features of human intracranial aneurysms. Several technical factors are critical for the successful induction of intracranial aneurysms in this model. Precise stereotaxic placement of the needle tip into the cerebrospinal fluid space is especially important. Aneurysm formations in this model can serve as a simple and easily interpretable outcome for future studies that utilize various inhibitors, knockout mice, or transgenic mice to test roles of specific molecules and pathways in the pathophysiology of intracranial aneurysms.
通过在高血压小鼠的右侧脑池向脑脊液中单次立体定向注射弹性蛋白酶，可诱发颅内动脉瘤。该小鼠模型在3 - 4周内会形成大型动脉瘤，发生率为60 - 80%。此模型中的颅内动脉瘤概括了人类颅内动脉瘤的关键病理特征。在该模型中成功诱发颅内动脉瘤，有几个技术因素至关重要。将针尖精确立体定向放置到脑脊液空间尤为重要。该模型中的动脉瘤形成可作为一个简单且易于解释的结果，用于未来利用各种抑制剂、基因敲除小鼠或转基因小鼠来测试特定分子和通路在颅内动脉瘤病理生理学中作用的研究。
