Ciszewicz Marta, Wu George, Tam Paul, Polubinska Alicja, Bre Borowicz Andrzej
Department of Pathophysiology, Poznan Medical School, Poznan, Poland.
Int J Artif Organs. 2011 Jun;34(6):489-94. doi: 10.5301/IJAO.2011.8467.
Preservation of the mesothelial cells (MCs) is crucial for longevity of the peritoneal dialysis membrane. Glucose accelerates aging of MC and we tested whether N-acetylglucosamine (NAG) has an identical effect.
Replicative aging of MCs was studied during 10 passages performed every three days in cells cultured in standard medium or in medium supplemented with Glucose 30 mmol/L or NAG 30 mmol/L. Changes in population doubling time and ß-galactosidase activity were used as an index of aging and compared with other cellular parameters.
Repeated passages of MC cause their aging, as reflected by prolongation of the population doubling time, increased ß-galactosidase activity, oxidative stress and release of cytokines. Healing of injured mesothelial monolayer is impaired in senescent cells. Glucose accelerates in vitro aging of MC, whereas NAG does not cause this effect.
Replacement of glucose with NAG in the dialysis fluid can slow down aging of MC.
间皮细胞（MCs）的保存对于腹膜透析膜的长期使用至关重要。葡萄糖会加速MC的衰老，我们测试了N-乙酰葡糖胺（NAG）是否有相同的作用。
在标准培养基或添加了30 mmol/L葡萄糖或30 mmol/L NAG的培养基中培养的细胞，每三天传代一次，共传代10次，研究MC的复制性衰老。将群体倍增时间和β-半乳糖苷酶活性的变化作为衰老指标，并与其他细胞参数进行比较。
MC的反复传代导致其衰老，表现为群体倍增时间延长、β-半乳糖苷酶活性增加、氧化应激和细胞因子释放。衰老细胞中受损间皮单层的愈合受损。葡萄糖会加速MC的体外衰老，而NAG不会产生这种作用。
在透析液中用NAG替代葡萄糖可以减缓MC的衰老。
