Breborowicz A, Kuzlan-Pawlaczyk M, Wieczorowska-Tobis K, Wisniewska J, Tam P, French I, Wu G
Department of Pathophysiology, Poznan Medical School, Poland.
Adv Perit Dial. 1998;14:31-5.
The effect of N-acetylglucosamine (NAG) on in vitro synthesis of glycosaminoglycans by human peritoneal mesothelial cells and fibroblasts was studied. In contrast to isosmotic concentrations of glucose, NAG increases the synthesis of hyaluronan by mesothelial cells and fibroblasts in a dose-dependent manner. This effect of NAG can be demonstrated in the presence of increased glucose levels in a medium, or in a medium mixed with effluent dialysate obtained from continuous ambulatory peritoneal dialysis (CAPD) patients. Glucose inhibits synthesis of sulphated glycosaminoglycans by peritoneal mesothelial cells and fibroblasts, whereas NAG stimulates their production. Our results demonstrate that NAG is an effective stimulator of the in vitro glycosaminoglycans synthesis by human peritoneal mesothelial cells and fibroblasts.
研究了N-乙酰葡糖胺（NAG）对人腹膜间皮细胞和成纤维细胞体外合成糖胺聚糖的影响。与等渗浓度的葡萄糖不同，NAG以剂量依赖的方式增加间皮细胞和成纤维细胞中透明质酸的合成。在培养基中葡萄糖水平升高时，或在与持续非卧床腹膜透析（CAPD）患者的流出透析液混合的培养基中，均可证明NAG的这种作用。葡萄糖抑制腹膜间皮细胞和成纤维细胞中硫酸化糖胺聚糖的合成，而NAG则刺激其产生。我们的结果表明，NAG是体外人腹膜间皮细胞和成纤维细胞合成糖胺聚糖的有效刺激剂。
