Barrera C N, Mazzolli A B, Colmerauer M E, Pasqualini C D
Allergol Immunopathol (Madr). 1978 Sep-Oct;6(5):403-8.
An experimental model was devised in which an AKR lymphoma is conditioned to grow in BALB mice leading to reproducible tumor incidence which makes tumor-bearing (progressor) and tumor-rejecting (regressor) animals simultaneously available. The object of this paper was to determine the effect of neonatal thymectomy (xT) on allogeneic tumor incidence and on anti-tumor cytophilic activity. The latter was determined by the adherence of lymphoma cells to guinea pig peritoneal macrophages previously incubated with preheated mouse serum. The results obtained, in 2-3 month old animals, show no difference in lethal tumor incidence between xT and intact mice, 36% (14/39) vs. 39% (14/36). Neither did xT alter the significant increase in cytophilic antibodies detected in regressor serum, 115 +/- 15 (S.E.) vs. 106+/- 22 0/00 macrophages bearing tumor cells as compared to control values in either xT or normal serum, 53 +/- 3 vs 52 +/- 3. This background cytophilic activity was not significantly altered in progressor serum of either xT or intact mice, 36 +/- 5 vs 65 +/- 6. The specificity of the antitumor cytophilic antibodies was determined by the negative results obtained when a different tumor was used as target cell. It can be concluded that ant-tumor cytophilic antibodies are detectable in regressor but not in progressor serum. Thymectomy in this model does not alter either in vivo tumor incidence or humoral cytophilic activity. Since no thymic remnants were encountered at autopsy, it is postulated that AKR lymphoma cells, which proved to be neoplastic T cells, are capable of rendering T-immunocompetent a thymectomized allogeneic BALB host.
设计了一种实验模型，在该模型中使AKR淋巴瘤适应于在BALB小鼠体内生长，从而产生可重复的肿瘤发生率，这使得同时获得荷瘤（进展者）和肿瘤排斥（消退者）动物成为可能。本文的目的是确定新生期胸腺切除（xT）对同种异体肿瘤发生率和抗肿瘤嗜细胞活性的影响。后者通过淋巴瘤细胞与预先用预热小鼠血清孵育的豚鼠腹腔巨噬细胞的黏附来确定。在2至3月龄动物中获得的结果显示，xT小鼠和完整小鼠之间的致死性肿瘤发生率没有差异，分别为36%（14/39）和39%（14/36）。xT也未改变在消退者血清中检测到的嗜细胞抗体的显著增加，与xT或正常血清中的对照值相比，携带肿瘤细胞的巨噬细胞分别为115±15（标准误）和106±22‰，而对照值分别为53±3和52±3。在xT或完整小鼠的进展者血清中，这种背景嗜细胞活性没有显著改变，分别为36±5和65±6。当使用不同肿瘤作为靶细胞时获得的阴性结果确定了抗肿瘤嗜细胞抗体的特异性。可以得出结论，在消退者血清中可检测到抗肿瘤嗜细胞抗体，而在进展者血清中则检测不到。在该模型中，胸腺切除既不改变体内肿瘤发生率，也不改变体液嗜细胞活性。由于尸检时未发现胸腺残余，因此推测被证明为肿瘤性T细胞的AKR淋巴瘤细胞能够使经胸腺切除的同种异体BALB宿主具有T免疫活性。
