Department of Life Sciences, Graduate School of Arts and Sciences, The University of Tokyo, 3-8-1 Komaba, Meguro-ku, Tokyo 153-8902, Japan.
Vaccine. 2011 Aug 26;29(37):6252-8. doi: 10.1016/j.vaccine.2011.06.073. Epub 2011 Jul 2.
One of the main hallmarks of Alzheimer's disease (AD) is senile plaques composed of amyloid β (Aβ). We developed a new edible vaccine: rice expressing GFP-Aβ42. In a previous report, we described the production of anti-Aβ antibodies in B6 mice fed Aβ rice mixed with cholera toxin B subunit (CTB). In this report, we investigated whether Aβ rice had therapeutic effects in the Tg2576 AD model mice. The anti-Aβ antibody titer was increased and levels of intracerebral Aβ (soluble and insoluble) and serum Aβ decreased. Because the value of IgG1/IgG2a in the Aβ feeding group was >1, immunization via Aβ rice may induce a non-inflammatory Th2 reaction. We also found that the Aβ vaccine improved memory, as assessed in a Y-maze test. The number of arm entries in the Y-maze test was lower in the Aβ feeding group than in the control group. These results suggest that the new edible vaccine Aβ rice may have therapeutic effects in AD.
阿尔茨海默病（AD）的主要特征之一是由淀粉样 β（Aβ）组成的老年斑。我们开发了一种新的可食用疫苗：表达 GFP-Aβ42 的水稻。在之前的报告中，我们描述了在喂食 Aβ 水稻和霍乱毒素 B 亚单位（CTB）的 B6 小鼠中产生抗 Aβ 抗体的情况。在本报告中，我们研究了 Aβ 水稻对 Tg2576 AD 模型小鼠是否具有治疗作用。抗 Aβ 抗体滴度增加，脑内 Aβ（可溶性和不溶性）和血清 Aβ 水平降低。由于 Aβ 喂养组的 IgG1/IgG2a 值>1，因此通过 Aβ 水稻进行免疫可能会引发非炎症性 Th2 反应。我们还发现 Aβ 疫苗改善了记忆，在 Y 迷宫测试中得到了评估。与对照组相比，Aβ 喂养组的 Y 迷宫测试中臂进入次数较少。这些结果表明，新型可食用疫苗 Aβ 水稻可能对 AD 具有治疗作用。
