Laboratory of Pharmaceutical Biotechnology, Faculty of Pharmaceutical Sciences, Ghent University, Harelbekestraat 72, 9000 Ghent, Belgium.
Forensic Sci Int Genet. 2012 Mar;6(2):258-62. doi: 10.1016/j.fsigen.2011.06.002. Epub 2011 Jul 2.
In forensic sciences, short tandem repeat (STR) analysis is a valuable tool in identifying the donor(s) of biological stains. Laser capture microdissection (LCM) can be used as a cell separating technique to isolate specific cell types in mixed samples. An important challenge lies in the development of a DNA isolation method appropriate for laser microdissected cells, as these samples usually contain minute amounts of cells. In this study three different DNA isolation methods for LCM collected cells were compared. The PicoPure DNA extraction method outperformed both other methods (IQ™ system and short alkaline method). Consequently, the minimal number of LCM collected cells necessary for STR typing was determined. Using the PicoPure DNA extraction method, full DNA profiles could be obtained from as little as 10 cells. Nevertheless, despite the occurrence of allelic drop out in some of the samples, lower amounts of cells gave rise to useful DNA profiles.
在法医学中，短串联重复序列（STR）分析是鉴定生物痕迹供体的有价值的工具。激光捕获显微切割（LCM）可用作细胞分离技术，以分离混合样本中的特定细胞类型。一个重要的挑战在于开发适用于激光微切割细胞的 DNA 分离方法，因为这些样本通常含有少量的细胞。在这项研究中，比较了三种不同的用于 LCM 收集细胞的 DNA 分离方法。PicoPure DNA 提取方法优于其他两种方法（IQ™系统和短碱法）。因此，确定了用于 STR 分型的最小数量的 LCM 收集细胞。使用 PicoPure DNA 提取方法，仅需 10 个细胞即可获得完整的 DNA 图谱。尽管在一些样本中出现等位基因丢失，但较少数量的细胞仍能产生有用的 DNA 图谱。
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