卵泡液中同型半胱氨酸和相关硫醇浓度的个体内变异性。
Intraindividual variability of homocysteine and related thiols concentrations in follicular fluid.
机构信息
Department of Biochemistry, Faculty of Medicine, Masaryk University, A16, Kamenice 5, Brno, Czech Republic.
出版信息
J Assist Reprod Genet. 2011 Sep;28(9):863-8. doi: 10.1007/s10815-011-9606-9. Epub 2011 Jul 5.
PURPOSE
To determine intraindividual variability in concentrations of homocysteine and related thiols in follicular fluids of particular follicles after ovarian stimulation and assess the differences between follicles with/or without oocytes.
METHODS
HPLC-FD analysis of plasma and follicular fluid cysteine, homocysteine, cysteinylglycine and glutathione in women undergoing IVF.
RESULTS
In blood plasma, the homocysteine, cysteine, and cysteinylglycine concentrations decreased significantly during stimulation with rec FSH (p<0.001). We found significant differences in concentrations of cysteine and glutathione between follicles with or without retrieved oocytes. High intraindividual variability in concentrations of thiols was determined.
CONCLUSIONS
The concentration variability of thiols between single follicles is very high and we recommend mean at least from 3 follicles with/or without oocytes for characterization of each woman. It is the best to examine individual follicles for further research and analysis of fertility outcomes.
目的
在卵巢刺激后确定特定卵泡的卵泡液中同型半胱氨酸和相关硫醇的个体内变异性，并评估有无卵母细胞的卵泡之间的差异。
方法
采用 HPLC-FD 分析接受 IVF 的女性的血浆和卵泡液半胱氨酸、同型半胱氨酸、半胱氨酰甘氨酸和谷胱甘肽。
结果
在血液血浆中，用重组 FSH 刺激期间，同型半胱氨酸、半胱氨酸和半胱氨酰甘氨酸浓度显著降低（p<0.001）。我们发现有或无取出卵母细胞的卵泡之间半胱氨酸和谷胱甘肽浓度存在显著差异。确定了硫醇浓度的个体内变异性很大。
结论
单个卵泡之间硫醇的浓度变异性非常高，我们建议至少从 3 个有/无卵母细胞的卵泡来描述每个女性。最好检查单个卵泡以进行进一步的研究和分析生育结果。
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