Cattaneo E, McKay R
Department of Brain and Cognitive, Massachusetts Institute of Technology, Cambridge 02139.
Nature. 1990 Oct 25;347(6295):762-5. doi: 10.1038/347762a0.
Nerve growth factor plays an important part in neuron-target interactions in the late embryonic and adult brain. We now report that this growth factor controls the proliferation of neuronal precursors in a defined culture system of cells derived from the early embryonic brain. Neuronal precursor cells were identified by expression of the intermediate filament protein nestin. These cells proliferate in response to nerve growth factor but only after they have been exposed to basic fibroblast growth factor. On withdrawal of nerve growth factor, the proliferative cells differentiate into neurons. Thus, in combination with other growth factors, nerve growth factor regulates the proliferation and terminal differentiation of neuroepithelial stem cells.
神经生长因子在胚胎后期和成年大脑的神经元与靶细胞相互作用中发挥重要作用。我们现在报告，这种生长因子在源自早期胚胎大脑的特定细胞培养系统中控制神经元前体细胞的增殖。通过中间丝蛋白巢蛋白的表达来鉴定神经元前体细胞。这些细胞在暴露于碱性成纤维细胞生长因子后，对神经生长因子有反应而增殖。去除神经生长因子后，增殖细胞分化为神经元。因此，神经生长因子与其他生长因子共同作用，调节神经上皮干细胞的增殖和终末分化。
