School of Physics and Astronomy, University of Southampton, Southampton SO17 1BJ, UK.
Langmuir. 2011 Aug 16;27(16):10119-23. doi: 10.1021/la2022177. Epub 2011 Jul 14.
Although carbodiimides and succinimides are broadly employed for the formation of amide bonds (i.e., in amino acid coupling), their use in the coupling of peptides to water-soluble carboxylic-terminated colloidal gold nanoparticles remains challenging. In this article, we present an optimization study for the successful coupling of the KPQPRPLS peptide to spherical and rodlike colloidal gold nanoparticles. We show that the concentration, reaction time, and chemical environment are all critical to achieving the formation of robust, peptide-coated colloidal nanoparticles. Agarose gel electrophoresis was used for the characterization of conjugates.
尽管碳二亚胺和琥珀酰亚胺被广泛用于酰胺键的形成（即在氨基酸偶联中），但它们在将肽偶联到水溶性羧酸末端胶体金纳米粒子上的应用仍然具有挑战性。在本文中，我们对成功将 KPQPRPLS 肽偶联到球形和棒状胶体金纳米粒子进行了优化研究。我们表明，浓度、反应时间和化学环境对于形成稳定的、肽包裹的胶体纳米粒子都是至关重要的。琼脂糖凝胶电泳用于偶联物的表征。
