Erasmus MC, Department of Medical Microbiology and Infectious Diseases, Rotterdam, The Netherlands.
Med Mycol. 2012 Feb;50(2):143-51. doi: 10.3109/13693786.2011.593005. Epub 2011 Jul 5.
Eumycetoma, a chronic granulomatous disease characterized by a subcutaneous mass, multiple sinuses and purulent discharge containing grains, remains difficult to diagnose and treat. Madurella mycetomatis is the most common causative agent of eumycetoma. Using a serum pool from patients with active mycetoma, we screened a M. mycetomatis-specific λgt11 cDNA library which was shown to contain 8% of cDNA inserts encoding proteins involved in glycolysis. Two of these enzymes, fructose-bisphosphate aldolase (FBA) and pyruvate kinase (PK), were produced in vitro and their antigenicity was studied with bead-based flow cytometry. It appeared that both FBA and PK IgG antibodies were present in eumycetoma patient sera. However, only FBA antibody levels were found to be significantly higher in eumycetoma patient sera when compared to healthy Sudanese controls. Furthermore, FBA and PK were also found to be expressed on the hyphae present in the mycetoma grain. In conclusion, this study presents two new antigenic proteins of M. mycetomatis next to the translationally controlled tumour protein (TCTP): the glycolytic enzymes FBA and PK. These antigens might be useful as vaccine-candidates in the prevention of mycetoma.
真菌性肿脚病是一种慢性肉芽肿性疾病，其特征为皮下肿块、多发性窦道和含有颗粒的脓性分泌物，目前仍难以诊断和治疗。枝顶孢霉是真菌性肿脚病最常见的病原体。我们利用来自活动性真菌肿脚病患者的血清池，筛选了一个枝顶孢霉特异性的 λgt11 cDNA 文库，该文库显示包含 8%编码糖酵解相关蛋白的 cDNA 插入物。这两种酶，果糖-1,6-二磷酸醛缩酶（FBA）和丙酮酸激酶（PK），在体外产生，并通过基于珠的流式细胞术研究了它们的抗原性。似乎 FBA 和 PK IgG 抗体都存在于真菌肿脚病患者的血清中。然而，与健康的苏丹对照组相比，只有 FBA 抗体水平在真菌肿脚病患者血清中被发现显著升高。此外，还发现 FBA 和 PK 也在真菌肿脚病颗粒中的菌丝上表达。总之，这项研究提出了两种新的枝顶孢霉抗原蛋白，除了翻译控制肿瘤蛋白（TCTP）之外：糖酵解酶 FBA 和 PK。这些抗原可能可用作真菌肿脚病预防的疫苗候选物。
