Sheng Xin, Zeng Hong, Zhang Meng, Yun Mi X, Yin Fei, Gu Fu K
School of Life Science, East China Normal University, Shanghai 200062, China.
Zoolog Sci. 2011 Jul;28(7):476-81. doi: 10.2108/zsj.28.476.
A recombinant plasmid L4440-GTU expressing γ-tubulin dsRNA of the ciliate Euplotes eurystomus was constructed and transferred into Escherichia coli strain HT115. The resultant E. coli bacteria were fed on E. eurystomus to inhibit the ciliate's γ-tubulin gene expression. As a result, the γ-tubulin gene expression level was decreased, and this inactivation blocked cell division, which was lethal. In addition, the loss of the C-tubule from the nine-microtubule triplets in basal bodies, and the disappearance of some microtubules or mislocalization of some microtubule organization units in the subpellicular microtubule layers were also observed. These results indicate that the γ-tubulin is not only important for the stability of the nine-microtubule triplets in basal bodies, but also necessary for the integrity of microtubule organization patterns in the subpellicular microtubule layer.
构建了表达纤毛虫阔口游仆虫γ-微管蛋白双链RNA的重组质粒L4440-GTU，并将其转入大肠杆菌HT115菌株。用所得大肠杆菌喂食阔口游仆虫以抑制其γ-微管蛋白基因表达。结果，γ-微管蛋白基因表达水平降低，这种失活阻断了细胞分裂，导致细胞死亡。此外，还观察到基体中九联微管三联体的C微管缺失，以及表膜下微管层中一些微管消失或一些微管组织单位定位错误。这些结果表明，γ-微管蛋白不仅对基体中九联微管三联体的稳定性很重要，而且对表膜下微管层中微管组织模式的完整性也是必需的。
