Cao Jiang, Li Xiu-Qin, Chen Chong, Zeng Ling-Yu, Cheng Hai, Li Zhen-Yu, Pang Xiu-Ying, Xu Kai-Lin
Laboratory of Hematology, Xuzhou Medical College Affiliated Hospital, Xuzhou 221002, Jiangsu Province, China.
Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2011 Jun;19(3):730-3.
The aim of this study was to investigate the expression of Th17 cells and regulatory T (Treg) cells in peripheral blood of patients with immune thrombocytopenia (ITP) and to clarify the role of the Th17/Treg cell ratio imbalance in pathogenesis of ITP. Patients were divided into the pre-treatment group (active group) (n = 38) and post-treatment group (remission group) according to the platelet count and curative effect. Post-treatment group was further divided into remission group (n = 24), partial remission group (n = 10), and non-remission group (n = 4). 30 healthy subjects were enrolled in control group. Flow cytometry was used to detect the percentages of peripheral blood Th17 cells and Treg cells in CD4(+) T cells from ITP patients and controls respectively. The results showed that the percentages of Th17 cells in active group and non-remission group were significantly higher than those in control group (p < 0.05). The percentages of Th17 cells in remission group, partial-remission group were also higher than those in control group, but there were no statistically significant differences between these groups. The percentage of Th17 cells in remission group was lower than that in active group, but there was also no statistically difference between two groups. The percentages of Treg cells in active group, partial-remission group and non-remission group significantly decreased, compared with in control group (p < 0.01). The percentage of Treg cells in remission group was lower than that in control group, but there was no statistically significant difference. The ratio of peripheral blood Th17/Treg cells in active group, partial-remission group and non-remission group was higher, as compared with in control group. The ratio of peripheral blood Th17/Treg cells in remission group was higher than that in control group, but there was no statistically difference between two groups. It is concluded the percentage of Th17 cells and the ratio of Th17/Treg cells are higher in active group. The percentage of Treg cells is low in active group, partial remission and non-remission groups. The imbalance of Th17/Treg ratio may play a critical role in ITP pathogenesis.
本研究旨在探讨免疫性血小板减少症（ITP）患者外周血中Th17细胞和调节性T（Treg）细胞的表达情况，并阐明Th17/Treg细胞比例失衡在ITP发病机制中的作用。根据血小板计数和疗效将患者分为预处理组（活动组）（n = 38）和治疗后组（缓解组）。治疗后组进一步分为缓解组（n = 24）、部分缓解组（n = 10）和未缓解组（n = 4）。30名健康受试者纳入对照组。采用流式细胞术分别检测ITP患者和对照组CD4(+) T细胞中外周血Th17细胞和Treg细胞的百分比。结果显示，活动组和未缓解组Th17细胞百分比显著高于对照组（p < 0.05）。缓解组、部分缓解组Th17细胞百分比也高于对照组，但这些组之间无统计学差异。缓解组Th17细胞百分比低于活动组，但两组之间也无统计学差异。与对照组相比，活动组、部分缓解组和未缓解组Treg细胞百分比显著降低（p < 0.01）。缓解组Treg细胞百分比低于对照组，但无统计学差异。与对照组相比，活动组、部分缓解组和未缓解组外周血Th17/Treg细胞比例更高。缓解组外周血Th17/Treg细胞比例高于对照组，但两组之间无统计学差异。结论是活动组Th17细胞百分比和Th17/Treg细胞比例更高。活动组、部分缓解组和未缓解组Treg细胞百分比低。Th17/Treg比例失衡可能在ITP发病机制中起关键作用。
