Uchima F D, Vallerga A K, Firestone G L, Bern H A
Department of Integrative Biology, University of California, Berkeley 94720.
Proc Soc Exp Biol Med. 1990 Nov;195(2):218-23. doi: 10.3181/00379727-195-43138.
Epithelia and fibromuscular walls were dissociated from the vaginae of ovariectomized BALB/cCrgl mice (ca. 41 days old) exposed neonatally to diethylstilbestrol (DES) or sesame oil and purified by centrifugation through Percoll density gradients. Neonatal exposure to DES caused the vaginal epithelium to become permanently proliferated and partially keratinized; the control epithelium was low cuboidal. The major cellular proteins expressed in each tissue compartment were examined by two-dimensional gel electrophoresis of [35S]methionine-labeled tissues. The epithelia and fibromuscular walls displayed distinctive two-dimensional protein patterns. In the DES-exposed vaginal epithelium, the expression of two proteins (one had a molecular size of 65 kDa and a pl of 6.0, and the other had a molecular size of 38 kDa and a pl of 6.3) was increased, while the expression of three proteins with molecular sizes of 25, 30, and 140 kDa and pls of 5.6, 5.6 and 6.7, respectively, was reduced, relative to the control epithelium. In the DES-exposed vaginal fibromuscular walls, the expression of 9 proteins was increased whereas the levels of 21 specific proteins, distinct from those in the epithelium, were decreased. Thus, long-term tissue-specific alterations in the synthesis of a select number of cellular proteins occur in the DES-exposed vagina.
将新生期暴露于己烯雌酚（DES）或芝麻油的去卵巢BALB/cCrgl小鼠（约41日龄）阴道的上皮和纤维肌壁分离，并通过Percoll密度梯度离心进行纯化。新生期暴露于DES会使阴道上皮永久性增殖并部分角化；对照上皮为低立方上皮。通过对[35S]甲硫氨酸标记的组织进行二维凝胶电泳，检测每个组织区室中表达的主要细胞蛋白。上皮和纤维肌壁呈现出独特的二维蛋白质图谱。与对照上皮相比，在暴露于DES的阴道上皮中，两种蛋白质（一种分子大小为65 kDa，等电点为6.0，另一种分子大小为38 kDa，等电点为6.3）的表达增加，而分子大小分别为25、30和140 kDa且等电点分别为5.6、5.6和6.7的三种蛋白质的表达减少。在暴露于DES的阴道纤维肌壁中，9种蛋白质的表达增加，而21种与上皮中不同的特异性蛋白质的水平降低。因此，在暴露于DES的阴道中，特定数量的细胞蛋白合成发生了长期的组织特异性改变。
