Zoological Pathology Program, University of Illinois, 2160 South First Street, Maywood, IL 60153, USA.
Vet Pathol. 2012 Sep;49(5):824-33. doi: 10.1177/0300985811412620. Epub 2011 Jul 5.
Captive cheetahs have an unusually severe progressive gastritis that is not present in wild cheetahs infected with the same strains of Helicobacter. This gastritis, when severe, has florid lymphocyte and plasma cell infiltrates in the epithelium and lamina propria with gland destruction, parietal cell loss, and, in some cases, lymphoid follicles. The local gastric immune response was characterized by immunohistochemistry in 21 cheetahs with varying degrees of gastritis. The character of the response was similar among types of gastritis except that cheetahs with severe gastritis had increased numbers (up to 70%) of lamina proprial CD79a+CD21- B cells. CD3+CD4+ T cells were present in the lamina propria, and CD3+CD8α+ T cells were within the glandular epithelium. Lymphoid aggregates had follicular differentiation with a central core of CD79a+/CD45R+ B cells and with an outer zone of CD3+ T cells that expressed both CD4 and CD8 antigens. MHC II antigens were diffusely expressed throughout the glandular and superficial epithelium. No cheetah had evidence of autoantibodies against the gastric mucosa when gastric samples from 30 cheetahs with different degrees of gastritis were incubated with autologous and heterologous serum. These findings indicate that T-cell distribution in cheetahs is qualitatively similar to that in other species infected with Helicobacter but that large numbers of lamina propria activated B cells and plasma cells did distinguish cheetahs with severe gastritis. Further research is needed to determine whether alterations in the Th1:Th2 balance are the cause of this more plasmacytic response in some cheetahs.
圈养猎豹患有一种异常严重的进行性胃炎，而在感染相同幽门螺杆菌菌株的野生猎豹中则不存在这种胃炎。这种胃炎在严重时，上皮和固有层会出现明显的淋巴细胞和浆细胞浸润，腺体破坏，壁细胞丧失，在某些情况下还会出现淋巴滤泡。对 21 只不同程度患有胃炎的猎豹进行了免疫组织化学分析，以研究其局部胃免疫反应。除了严重胃炎的猎豹固有层 CD79a+CD21- B 细胞数量（高达 70%）增加外，不同类型胃炎的反应特征相似。固有层存在 CD3+CD4+ T 细胞，腺体内存在 CD3+CD8α+ T 细胞。淋巴聚集物具有滤泡分化特征，中央核心为 CD79a+/CD45R+ B 细胞，周围为表达 CD4 和 CD8 抗原的 CD3+ T 细胞。MHC II 抗原在整个腺体和表面上皮均有弥漫表达。当对 30 只不同程度患有胃炎的猎豹的胃样本进行孵育，并用自身和异体血清进行孵育时，没有一只猎豹显示出针对胃黏膜的自身抗体。这些发现表明，猎豹的 T 细胞分布在质上与其他感染幽门螺杆菌的物种相似，但大量固有层激活的 B 细胞和浆细胞确实可以区分患有严重胃炎的猎豹。需要进一步研究以确定 Th1:Th2 平衡的改变是否是某些猎豹产生这种更多浆细胞反应的原因。
