Kong D Y, Wang Z L, Lin C K, Quan Z W, Li Y Y, Li C X, Lin J
Key Laboratory of Rare Earth Chemistry and Physics, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, People's Republic of China. Graduate School of the Chinese Academy of Sciences, Beijing 100049, People's Republic of China.
Nanotechnology. 2007 Feb 21;18(7):075601. doi: 10.1088/0957-4484/18/7/075601. Epub 2007 Jan 12.
CeF(3):Tb(3+) nanoparticles (short pillar-like morphology with an average length and width of 11 and 5 nm, respectively) were successfully prepared by a polyol process using diethyleneglycol (DEG) as solvent. After being functionalized with a SiO(2)-NH(2) layer, these CeF(3):Tb(3+) nanoparticles can be conjugated with biotin molecules (activated by thionyl chloride) and further with avidin. The as-formed CeF(3):Tb(3+) nanoparticles, CeF(3):Tb(3+) nanoparticles functionalized with amino groups, biotin conjugated amino-functionalized CeF(3):Tb(3+) nanoparticles and biotinylated CeF(3):Tb(3+) nanoparticles bonded with avidin were characterized by x-ray diffraction (XRD), transmission electron microscopy (TEM), Fourier transform infrared (FT-IR), UV/vis absorption spectra and luminescence spectra, respectively. The biofunctionalization of the CeF(3):Tb(3+) nanoparticles has less effect on their luminescence properties, i.e. they still show strong green emission (from Tb(3+), with (5)D(4)-(7)F(5) at 543 nm as the most prominent group), indicative of the great potential for these CeF(3):Tb(3+) nanoparticles to be used as biological fluorescence probes.
采用以二甘醇（DEG）为溶剂的多元醇法成功制备了CeF(3):Tb(3+)纳米颗粒（短柱状形态，平均长度和宽度分别为11纳米和5纳米）。在用SiO(2)-NH(2)层进行功能化后，这些CeF(3):Tb(3+)纳米颗粒可与生物素分子（由亚硫酰氯活化）共轭，进而与抗生物素蛋白结合。分别通过X射线衍射（XRD）、透射电子显微镜（TEM）、傅里叶变换红外光谱（FT-IR）、紫外/可见吸收光谱和发光光谱对所形成的CeF(3):Tb(3+)纳米颗粒、氨基功能化的CeF(3):Tb(3+)纳米颗粒、生物素共轭氨基功能化的CeF(3):Tb(3+)纳米颗粒以及与抗生物素蛋白结合的生物素化CeF(3):Tb(3+)纳米颗粒进行了表征。CeF(3):Tb(3+)纳米颗粒的生物功能化对其发光性能影响较小，即它们仍显示出强烈的绿色发射（来自Tb(3+)，以543纳米处的(5)D(4)-(7)F(5)作为最突出的峰），这表明这些CeF(3):Tb(3+)纳米颗粒作为生物荧光探针具有巨大潜力。
