Liu Jian Xin, Liu Yong, Tang Feng Ru
Institute of Neurobiology, School of Medicine, Xi'an Jiaotong University, Xi'an, P.R. China.
Acta Neurobiol Exp (Wars). 2011;71(2):220-32. doi: 10.55782/ane-2011-1842.
We investigated the protein expression of different protein kinase C (PKC) isoforms (PKC-alpha, PKC-beta1, PKC-beta2, PKC-gamma, PKC-delta, PKC-epsilon, PKC-eta and PKC-zeta) in the hippocampus of normal control mice and progressive changes in PKC isoforms expression during and after pilocarpine induced status epilepticus (PISE). We showed the reduced expression of PKC-delta, PKC-eta and PKC-zeta in interneurons in the CA1 area and in the hilus of the dentate gyrus during or after PISE. Increased expression of PKC-alpha and PKC-beta1 was demonstrated in the stratum pyramidale of CA3 area, and PKC-epsilon was up-regulated in the stratum lucidum of the CA3 area during or after PISE. Our results suggest that hippocampal PKC isoforms may play different roles in seizure generation, and be targets for development of anti-convulsive drugs.
我们研究了正常对照小鼠海马中不同蛋白激酶C（PKC）亚型（PKC-α、PKC-β1、PKC-β2、PKC-γ、PKC-δ、PKC-ε、PKC-η和PKC-ζ）的蛋白表达，以及毛果芸香碱诱导的癫痫持续状态（PISE）期间及之后PKC亚型表达的渐进性变化。我们发现，在PISE期间或之后，CA1区中间神经元以及齿状回门区中PKC-δ、PKC-η和PKC-ζ的表达降低。在PISE期间或之后，CA3区锥体层中PKC-α和PKC-β1的表达增加，并且CA3区透明层中PKC-ε上调。我们的结果表明，海马PKC亚型可能在癫痫发作的产生中发挥不同作用，并且可能成为抗惊厥药物开发的靶点。
