Marica D, Kadar I, Rudăreanu N, Kadar L, Corlăţean M, Miclea I, Boia G E
Facultatea de medicină veterinară, Cluj-Napoca.
Rev Ig Bacteriol Virusol Parazitol Epidemiol Pneumoftiziol Bacteriol Virusol Parazitol Epidemiol. 1990 Jan-Mar;35(1):71-6.
The development of the methyl red reaction (MR) in Klebsielleae, analyzed during a 4-day thermostatting, showed important pH-variations. Two steps are distinguished during the reaction dynamics: the former of acidification, common to all Enterobacteriaceae and the latter of alkaline reversion, specific to Enterobacter, Klebsiella, Serratia and other MR germs. The processes determining the alkaline reversion are conditioned by the presence of large amounts of air/oxygen (aerobic processes). In this situation, the lack of oxygen and maintenance of a relative degree of anaerobiosis block realkalinization (media covered with paraffin oil) whereas the large aeration stimulates it (optimum ratio between the large contact surface and a small volume of medium). Alkaline reversion cannot be explained by the more or less intense process of glucose fermentation but by degradations of nitrogenized substances with formation of amines, ammonia, and other basic compounds. That is why the analysis of the reaction mechanism of methyl red has to consider the characteristics of the catabolism of the nitrogenized substances and the intense realkalinization capacity of the culture medium proper to Klebsielleae but poorly expressed and non efficient in Enterobacteriaceae.
在4天恒温培养过程中对克雷伯菌属中甲基红反应（MR）的发展进行分析，结果显示出重要的pH值变化。在反应动力学过程中可区分出两个阶段：第一个阶段是酸化，这是所有肠杆菌科细菌共有的；第二个阶段是碱性逆转，这是肠杆菌属、克雷伯菌属、沙雷菌属及其他MR阳性菌所特有的。决定碱性逆转的过程受大量空气/氧气（需氧过程）的存在所制约。在这种情况下，缺氧以及维持相对程度的厌氧状态会阻止碱化（培养基用石蜡油覆盖），而大量通气则会刺激碱化（大的接触表面积与小体积培养基之间的最佳比例）。碱性逆转不能用葡萄糖发酵的或多或少的强烈过程来解释，而是由含氮物质降解形成胺、氨和其他碱性化合物来解释。这就是为什么对甲基红反应机制的分析必须考虑含氮物质分解代谢的特点以及克雷伯菌属所特有的培养基的强烈碱化能力，而这种能力在肠杆菌科中表现较弱且效率不高。
