Ala-Kapee M, Nevanlinna H, Mali M, Jalkanen M, Schröder J
Department of Genetics, University of Helsinki, Finland.
Somat Cell Mol Genet. 1990 Sep;16(5):501-5. doi: 10.1007/BF01233200.
Syndecan, a cell surface proteoglycan, is an integral membrane protein acting as a receptor for the extracellular matrix. For chromosomal localization of the human syndecan gene, a panel of mouse-human somatic cell hybrids was analyzed by Southern blotting using the cDNA probe for human syndecan. The hybrids were karyotyped at the time of DNA extraction. A band corresponding to the human syndecan gene in Southern blots was found only in a hybrid cell line containing human chromosome 2. This hybrid was subcloned and its subclones were analyzed by Southern blotting and karyotyped. Subclones carrying human chromosome 2 contained the syndecan gene, while subclones not carrying this chromosome did not. The human syndecan gene is thus assigned to chromosome 2.
Syndecan是一种细胞表面蛋白聚糖，是一种作为细胞外基质受体的整合膜蛋白。为了对人类Syndecan基因进行染色体定位，使用人类Syndecan的cDNA探针通过Southern印迹法分析了一组小鼠-人类体细胞杂种。在提取DNA时对杂种进行了核型分析。在Southern印迹中，仅在含有人类2号染色体的杂种细胞系中发现了与人类Syndecan基因相对应的条带。对该杂种进行了亚克隆，并通过Southern印迹法对其子克隆进行了分析并进行了核型分析。携带人类2号染色体的亚克隆含有Syndecan基因，而不携带该染色体的亚克隆则没有。因此，人类Syndecan基因被定位到2号染色体上。
