Plant Physiology, Biological and Geological Sciences, Faculty of Education, Ain Shams University, El Makres St. Roxy, Cairo, Egypt.
Protoplasma. 2012 Jul;249(3):625-35. doi: 10.1007/s00709-011-0300-7. Epub 2011 Jul 6.
Soil salinity is one of the most severe factors limiting growth and physiological response in Vigna sinensis plants. Plant salt stress tolerance requires the activation of complex metabolic activities including antioxidative pathways, especially reactive oxygen species and scavenging systems within the cells which can contribute to continued growth under water stress. The present investigation was carried out to study the role of brassinolide in enhancing tolerance of cowpea plants to salt stress (NaCl). Treatment with 0.05 ppm brassinolide as foliar spray mitigated salt stress by inducing enzyme activities responsible for antioxidation, e.g., superoxide dismutase, peroxidase, polyphenol oxidase, and detoxification as well as by elevating contents of ascorbic acid, tocopherol, and glutathione. On the other hand, total soluble proteins decreased with increasing NaCl concentrations in comparison with control plants. However, lipid peroxidation increased with increasing concentrations of NaCl. In addition to, the high concentrations of NaCl (100 and 150 mM) decreased total phenol of cowpea plants as being compared with control plants. SDS-PAGE of protein revealed that NaCl treatments alone or in combination with 0.05 ppm brassinolide were associated with the disappearance of some bands or appearance of unique ones in cowpea plants. Electrophoretic studies of α-esterase, β-esterase, polyphenol oxidase, peroxidase, acid phosphatase, and superoxide dismutase isoenzymes showed wide variations in their intensities and densities among all treatments.
土壤盐度是限制豇豆属植物生长和生理反应的最严重因素之一。植物耐盐性需要激活复杂的代谢活动，包括抗氧化途径，特别是细胞内的活性氧和清除系统，这些系统有助于在水分胁迫下继续生长。本研究旨在研究油菜素内酯在增强豇豆植物耐盐性（NaCl）中的作用。叶面喷施 0.05ppm 油菜素内酯可通过诱导与抗氧化有关的酶活性（如超氧化物歧化酶、过氧化物酶、多酚氧化酶和解毒作用）以及提高抗坏血酸、生育酚和谷胱甘肽的含量来减轻盐胁迫。另一方面，与对照植物相比，随着 NaCl 浓度的增加，总可溶性蛋白质含量降低。然而，随着 NaCl 浓度的增加，脂质过氧化作用增强。此外，与对照植物相比，高浓度的 NaCl（100 和 150mM）会降低豇豆植物的总酚含量。蛋白质的 SDS-PAGE 显示，单独用 NaCl 处理或与 0.05ppm 油菜素内酯联合处理会导致豇豆植物中一些条带消失或出现独特条带。α-酯酶、β-酯酶、多酚氧化酶、过氧化物酶、酸性磷酸酶和超氧化物歧化酶同工酶的电泳研究表明，所有处理之间它们的强度和密度都有很大的变化。
