Kostopoulou Zacharoula, Therios Ioannis, Molassiotis Athanassios
Laboratory of Pomology, Department of Horticulture, School of Agriculture, Aristotle University of Thessaloniki, 541 24 Thessaloniki, Greece.
Laboratory of Pomology, Department of Horticulture, School of Agriculture, Aristotle University of Thessaloniki, 541 24 Thessaloniki, Greece.
Plant Physiol Biochem. 2014 May;78:1-9. doi: 10.1016/j.plaphy.2014.02.011. Epub 2014 Feb 20.
Resveratrol, a phytoalexin found in red wine, has the potential to impact a variety of human diseases but its function in plants exposed to stressful conditions is still unknown. In the present study the effect of exogenous application of resveratrol (Res), α-tocopherol (α-Toc) and their combination (Res+α-Toc) in salt adaptation of citrus seedlings was investigated. It was found that Res, α-Toc or Res+α-Toc treatments reduced NaCl-derived membrane permeability (EL), lipid peroxidation (MDA) and pigments degradation, whereas companied Res and α-Toc application also reduced H2O2 accumulation in leaves and restored the reduction of photosynthesis induced by NaCl. Application of Res under salinity retained Cl- in roots while Res+α-Toc reduced the translocation of Na+ and Cl- to leaves. Carbohydrates and proline, phenols, total ascorbic acid and glutathione were remarkably affected by NaCl as well as by chemical treatments in leaves and roots of citrus. NaCl treatment increased the activity of superoxide dismutase (SOD), ascorbate peroxidase (APX), peroxidase (POD), glutathione reductase (GR), polyphenol oxidase (PPO) in leaves while SOD and POD activities were decreased in roots by this treatment. Also, Res, α-Toc or Res+α-Toc treatments displayed tissue specific activation or deactivation of the antioxidant enzymes. Overall, this work revealed a new functional role of Res in plants and provided evidence that the interplay of between Res and α-Toc is involved in salinity adaptation.
白藜芦醇是一种存在于红酒中的植保素，它有可能影响多种人类疾病，但其在遭受胁迫条件的植物中的功能仍不清楚。在本研究中，研究了外源施加白藜芦醇（Res）、α-生育酚（α-Toc）及其组合（Res+α-Toc）对柑橘幼苗盐分适应的影响。结果发现，Res、α-Toc或Res+α-Toc处理降低了NaCl诱导的膜通透性（EL）、脂质过氧化（MDA）和色素降解，而同时施加Res和α-Toc还减少了叶片中H2O2的积累，并恢复了由NaCl诱导的光合作用降低。在盐分胁迫下施加Res可使Cl-保留在根部，而Res+α-Toc则减少了Na+和Cl-向叶片的转运。碳水化合物、脯氨酸、酚类、总抗坏血酸和谷胱甘肽在柑橘的叶片和根部均受到NaCl以及化学处理的显著影响。NaCl处理增加了叶片中超氧化物歧化酶（SOD）、抗坏血酸过氧化物酶（APX）、过氧化物酶（POD）、谷胱甘肽还原酶（GR）、多酚氧化酶（PPO）的活性，而该处理使根部的SOD和POD活性降低。此外，Res、α-Toc或Res+α-Toc处理对抗氧化酶表现出组织特异性的激活或失活作用。总体而言，这项工作揭示了Res在植物中的一种新功能作用，并提供了证据表明Res和α-Toc之间的相互作用参与了盐分适应过程。
