Wada Shinya, Ode Hirotaka, Hobo Seiji, Niwa Hidekazu, Katayama Yoshinari, Takatori Kosuke
Racehorse Hospital, Ritto Training Center, The Japan Racing Association, 1028, Misono, Ritto, Shiga 520-3085, Japan.
Vet Ophthalmol. 2011 Jul;14(4):267-70. doi: 10.1111/j.1463-5224.2011.00891.x. Epub 2011 Apr 19.
To describe a case of superficial keratomycosis caused by Mortierella wolfii (M. wolfii) in a horse.
A thoroughbred filly was presented with painful right eye of 2 days' duration. A superficial corneal ulcer was observed ventrally together with multifocal punctuate opacities axially. Samples were collected by swabbing and scraping the ulcerated lesion and submitted for microbiologic and cytologic examination.
Microscopic evaluation of debrided corneal tissue revealed the presence of nonseptate fungal hyphae, and culture of a corneal swab yielded fungal growth. Medical treatment with topical antifungal, antibiotic and autogenous serum and systemic anti-inflammatory resolved the problem within 2 weeks.
Cytologic evaluation of a corneal scraping was useful to make a clinical diagnosis of keratomycosis. Based on the mycological characteristics, the fungus isolated from the corneal lesion was identified as M. wolfii. To the authors' knowledge, this is the first case report of equine keratomycosis associated with this fungus, although the organism is known to infect various organs of cattle.
描述一匹马由沃尔夫被孢霉（M. wolfii）引起的浅表角膜真菌病病例。
一匹纯种小母马因右眼疼痛2天前来就诊。在角膜腹侧观察到一个浅表角膜溃疡，同时在角膜轴向观察到多灶性点状混浊。通过擦拭和刮取溃疡病变部位采集样本，并进行微生物学和细胞学检查。
对清创后的角膜组织进行显微镜评估发现存在无隔真菌菌丝，角膜拭子培养有真菌生长。局部使用抗真菌药、抗生素和自体血清以及全身抗炎治疗在2周内解决了问题。
角膜刮片的细胞学评估有助于角膜真菌病的临床诊断。根据真菌学特征，从角膜病变分离出的真菌被鉴定为沃尔夫被孢霉。据作者所知，这是首例与该真菌相关的马角膜真菌病病例报告，尽管已知该病原体可感染牛的各个器官。
