Papapdopoulos V, Kamtchouing P, Boujrad N, Pisselet C, Perreau C, Locatelli A, Drosdowsky M A, Hochereau de Reviers M T, Carreau S
Laboratoire de biochimie, URA CNRS 609, CHU Côte de Nacre, Caen, France.
Acta Endocrinol (Copenh). 1990 Sep;123(3):345-52. doi: 10.1530/acta.0.1230345.
Intracellular cyclic AMP and testosterone productions by purified mature rat Leydig cells were stimulated by oLH (25 micrograms/l) 18- and 12-fold, respectively, after a 5-h incubation period. The replacement of the incubation medium by charcoal-treated testicular venous plasma (40%, v/v) from adult rams in the breeding season induced an inhibition of cyclic AMP and testosterone productions (82 and 66%, respectively, of oLH-stimulated values). Testicular arterial plasma is less effective than testicular venous plasma, even when it originates from non-breeding season rams; in that case, testicular venous and arterial plasma strongly inhibit testosterone productions (84 and 67%, respectively of oLH-stimulated values), which probably indicates that the inhibitory activity is higher in the non-breeding season. The addition of peripheral plasma leads to a testosterone production equal to 35 and 65% of the oLH-stimulated values, respectively, for ram blood collected in non-breeding and breeding seasons. The same concentration of ovine testicular lymph or rete testis fluid is without significant effect on cyclic AMP production; however, testosterone is slightly decreased by lymph but enhanced by rete testis fluid. Increasing amounts of venous or arterial testicular blood induce a dose-related decrease of the specific binding of labelled hCG in both rat and ram testicular membranes. This inhibiting factor is present in peripheral and testicular blood of either control or hypophysectomized or castrated rams, is a protein in nature, heat-sensitive, and has an apparent molecular weight higher than 10,000 daltons.(ABSTRACT TRUNCATED AT 250 WORDS)
经5小时孵育后，纯化的成年大鼠睾丸间质细胞产生的细胞内环状AMP和睾酮分别被促黄体生成素（oLH，25微克/升）刺激了18倍和12倍。在繁殖季节，用经活性炭处理的成年公羊睾丸静脉血浆（40%，v/v）替换孵育培养基，可抑制环状AMP和睾酮的产生（分别为oLH刺激值的82%和66%）。睾丸动脉血浆的作用比睾丸静脉血浆弱，即使它来自非繁殖季节的公羊；在这种情况下，睾丸静脉和动脉血浆强烈抑制睾酮的产生（分别为oLH刺激值的84%和67%），这可能表明非繁殖季节的抑制活性更高。添加外周血浆后，非繁殖季节和繁殖季节采集的公羊血液中睾酮的产生量分别相当于oLH刺激值的35%和65%。相同浓度的绵羊睾丸淋巴液或睾丸网液对环状AMP的产生没有显著影响；然而，睾酮会因淋巴液而略有下降，但会因睾丸网液而增加。大鼠和公羊睾丸膜中，越来越多的睾丸静脉血或动脉血会导致标记的人绒毛膜促性腺激素（hCG）特异性结合呈剂量相关下降。这种抑制因子存在于对照、垂体切除或阉割公羊的外周血和睾丸血中，本质上是一种蛋白质，对热敏感，表观分子量高于10,000道尔顿。（摘要截选至250字）
