Department of Cardiology, First Affiliated Hospital of Xinjiang Medical University, Urumqi, PR China.
Clin Appl Thromb Hemost. 2012 Jan-Feb;18(1):72-8. doi: 10.1177/1076029611412365. Epub 2011 Jul 6.
Matrix metalloproteinase 9 (MMP-9) plays a pivotal role in early atherosclerosis, vascular remodeling, and development of arterial plaque rupture. The potentially functional MMP-9 gene polymorphism may contribute to the susceptibility of myocardial infarction (MI). We aimed to investigate the association between the interaction of 2 single-nucleotide polymorphisms ([SNPs] -1562C>T and R279Q) of the MMP-9 gene and smoking with MI in a Uighur population of China.
A case-control study composed of 384 coronary angiography proven patients with MI and 451 sex-matched and ethnically matched control participants were enrolled in the study. The genotypes of the 2 selected SNPs were determined by the method of polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP).
Analysis of 2 SNPs, the frequency of -1562 CT and TT genotypes in MI group was significantly higher than in control group (25.52% vs 17.29%; P = .004). And the -1562 T allele showed significant association with MI (MI vs controls: 14.19% vs 9.31%; P = .002). R279Q locus did not show significant differences between patients and controls ( P > .05). Multiple logistic regression analysis showed that -1562 T allele (CT or TT genotype; odds ratio [OR] 1.31, 95% confidence interval [CI] 1.22-1.40; P = .004), the interaction between -1562 T allele and smoking (OR 4.42, 95% CI 2.74-7.13; P < .001), and the interaction between 279 Q allele and smoking (OR 2.07, 95% CI: 1.04-4.13; P = .021) were independent risk factors for MI.
There findings suggest that MMP-9 -1562C>T polymorphism could be associated with the susceptibility to MI. Another SNP (R279Q) polymorphism was not significantly associated with MI. The -1562C>T or R279Q polymorphism of MMP-9 gene and smoking have a synergistic effect and are significantly associated with the risk of MI in Chinese Uighur population, respectively.
基质金属蛋白酶 9（MMP-9）在早期动脉粥样硬化、血管重塑和动脉斑块破裂的发展中起着关键作用。潜在功能的 MMP-9 基因多态性可能导致心肌梗死（MI）的易感性。我们旨在研究中国维吾尔族人群中 MMP-9 基因的 2 个单核苷酸多态性（[SNPs] -1562C>T 和 R279Q）与吸烟的相互作用与 MI 之间的关系。
这项病例对照研究纳入了 384 例经冠状动脉造影证实的 MI 患者和 451 名性别和种族匹配的对照组参与者。采用聚合酶链反应和限制性片段长度多态性（PCR-RFLP）法确定 2 个所选 SNP 的基因型。
对 2 个 SNP 进行分析，MI 组-1562 CT 和 TT 基因型的频率明显高于对照组（25.52%比 17.29%；P=0.004）。-1562 T 等位基因与 MI 显著相关（MI 与对照组：14.19%比 9.31%；P=0.002）。R279Q 位点在患者和对照组之间无显著差异（P＞0.05）。多因素 logistic 回归分析显示，-1562 T 等位基因（CT 或 TT 基因型；比值比[OR]1.31，95%置信区间[CI]1.22-1.40；P=0.004）、-1562 T 等位基因与吸烟的相互作用（OR 4.42，95%CI 2.74-7.13；P＜0.001）和 279 Q 等位基因与吸烟的相互作用（OR 2.07，95%CI：1.04-4.13；P=0.021）是 MI 的独立危险因素。
研究结果表明，MMP-9 -1562C>T 多态性可能与 MI 的易感性有关。另一个 SNP（R279Q）多态性与 MI 无显著相关性。MMP-9 基因的-1562C>T 或 R279Q 多态性与吸烟有协同作用，分别与中国维吾尔族人群 MI 的发病风险显著相关。
