Department of Microelectronics, Bio-devices group, Delft University of Technology/DIMES, Mekelweg 4, 2628 CD, The Netherlands.
Lab Chip. 2011 Aug 21;11(16):2711-9. doi: 10.1039/c1lc20075e. Epub 2011 Jul 6.
Fabrication of nanochannels is drawing considerable interest due to its broad applications in nanobiotechnology (e.g. biomolecular sensing and single DNA manipulation). Nanochannels offer distinct advantages in allowing a slower translocation and multiple sensing spots along the channel, both of which improve the read-out resolution. However, implementing electrodes inside the nanochannel has rarely been demonstrated to our knowledge. The device described in this work is a Si-Glass anodically bonded Lab-on-a-Chip (LOC) device of a few millimetres in size capable of performing DNA manipulation. The LOC device structure is based on two mainstream microchannels interconnected by nanochannels. DNA, once trapped within the nanochannel, has been tracked throughout the length of the channel and the data have been recorded and analysed.
由于纳米通道在纳米生物技术（例如生物分子传感和单 DNA 操作）中的广泛应用，其制造引起了相当大的关注。纳米通道具有允许较慢的易位和沿通道的多个传感点的独特优势，这两者都提高了读出分辨率。然而，据我们所知，在纳米通道内实现电极的情况很少见。本文所述的器件是一种 Si-玻璃阳极键合的微流控芯片（LOC）器件，尺寸为数毫米，能够进行 DNA 操作。LOC 器件结构基于两个主流微通道，通过纳米通道相互连接。DNA 一旦被困在纳米通道内，就会在整个通道长度内被跟踪，并记录和分析数据。
