Jiangxi Key Laboratory for Mass Spectrometry and Instrumentation, Department of Applied Chemistry, East China Institute of Technology, Fuzhou, Jiangxi 344000, P. R. China.
Analyst. 2011 Sep 21;136(18):3599-601. doi: 10.1039/c1an15410a. Epub 2011 Jul 6.
The high-throughput and sensitive characterization of native proteins in biological samples is of increasing interest in multiple disciplines. Extractive electrospray ionization (EESI) forms ions of native proteins including lysozyme, α-chymotrypsin, myoglobin, human serum albumin, RNAse A and blood hemoglobin in extremely complex biosamples or PBS buffer solutions by softly depositing charges on the protein molecules. This method produces no significant conformational changes of the proteins in the ion formation process, and features direct detection of trace proteins present in biological matrices. The detection limit of low pmol L(-1) for lysozyme in untreated biological liquids such as human urine and tears was demonstrated using EESI mass spectrometry (MS), showing an attractive MS platform for the direct analysis of native proteins in actual biological samples.
在多个学科领域，高通量且灵敏地对生物样本中的天然蛋白质进行特征分析正受到越来越多的关注。萃取式电喷雾离子化（EESI）通过在蛋白质分子上柔和地沉积电荷，从而使溶菌酶、α-糜蛋白酶、肌红蛋白、人血清白蛋白、RNAse A 和血血红蛋白等天然蛋白质及其它极为复杂的生物样品或 PBS 缓冲溶液中的离子化。该方法在蛋白质的离子形成过程中不会导致显著的构象变化，且具有直接检测生物基质中痕量蛋白质的特点。EESI 质谱（MS）检测结果表明，未经处理的生物液体（如人尿和眼泪）中的溶菌酶检测限低至 pmol L(-1)，这表明 EESI 是一种用于直接分析实际生物样本中天然蛋白质的极具吸引力的 MS 平台。
