National Institute for Environmental Studies, Onogawa 16-2, Tsukuba, Ibaraki, 305-0053, Japan.
Appl Microbiol Biotechnol. 2012 Jan;93(2):847-57. doi: 10.1007/s00253-011-3445-6. Epub 2011 Jul 7.
The microbial mats responsible for biological desulfurization from biogas in a full-scale anaerobic digester were characterized in terms of their structure, as well as their chemical and microbial properties. Filament-shaped elemental sulfur 100-500 μm in length was shown to cover the mats, which cover the entire headspace of the digester. This is the first report on filamentous sulfur production in a non-marine environment. The results of the analysis of the mats suggest that the key players in the sulfide oxidation and sulfur production in the bio-desulfurization in the headspace of the digester were likely to be two sulfide-oxidizing bacteria (SOB) species related to Halothiobacillus neapolitanus and Sulfurimonas denitrificans, and that the microbial community, cell density, activity for sulfide oxidation varied according to the environmental conditions at the various locations of the mats. Since the water and nutrients necessary for the SOB were provided by the digested sludge droplets deposited on the mats, and our results show that a higher rate of sulfide oxidation occurred with more frequent digested sludge deposition, the habitat of the SOB needs to be made in the lower part of the headspace near the liquid level of the digested sludge to maintain optimal conditions.
对来自大规模厌氧消化器中沼气的生物脱硫的微生物垫进行了特征描述，包括其结构以及化学和微生物特性。长 100-500μm 的丝状元素硫被证明覆盖了整个消化器顶部空间的垫子。这是在非海洋环境中丝状硫生产的第一个报告。对垫子的分析结果表明，在消化器顶部空间的生物脱硫中，硫化物氧化和硫生产的关键参与者可能是两种与那不勒斯硫杆菌和脱氮硫杆菌有关的硫化物氧化细菌（SOB）物种，并且微生物群落、细胞密度、硫化物氧化活性根据垫子各个位置的环境条件而变化。由于为 SOB 提供了所需的水和养分的消化污泥滴沉积在垫子上，并且我们的结果表明，随着消化污泥沉积更加频繁，硫化物氧化的速率更高，因此需要在靠近消化污泥液位的头空间下部为 SOB 创造栖息地，以维持最佳条件。
