Yang Zhendong, Liu Zhenghua, Sklodowska Aleksandra, Musialowski Marcin, Bajda Tomasz, Yin Huaqun, Drewniak Lukasz
Institute of Microbiology, Faculty of Biology, University of Warsaw, Miecznikowa 1, 02-096 Warsaw, Poland.
School of Minerals Processing and Bioengineering, Central South University, No. 932 Lushan South Road, Changsha 410083, China.
Microorganisms. 2021 Mar 16;9(3):611. doi: 10.3390/microorganisms9030611.
Management of excessive aqueous sulfide is one of the most significant challenges of treating effluent after biological sulfate reduction for metal recovery from hydrometallurgical leachate. The main objective of this study was to characterize and verify the effectiveness of a sulfide-oxidizing bacterial (SOB) consortium isolated from post-mining wastes for sulfide removal from industrial leachate through elemental sulfur production. The isolated SOB has a complete sulfur-oxidizing metabolic system encoded by genes and is dominated by the genus. XRD analysis confirmed the presence of elemental sulfur in the collected sediment during cultivation of the SOB in synthetic medium under controlled physicochemical conditions. The growth yield after three days of cultivation reached 2.34 g/mol, while approximately 84% of sulfide was transformed into elemental sulfur after 5 days of incubation. Verification of isolated SOB on the industrial effluent confirmed that it can be used for effective sulfide concentration reduction (100% reduced from the initial 75.3 mg/L), but for complete leachate treatment (acceptable for discharged limits), bioaugmentation with other bacteria is required to ensure adequate reduction of chemical oxygen demand (COD).
过量硫化物的管理是处理生物硫酸盐还原后废水以从湿法冶金浸出液中回收金属的最重大挑战之一。本研究的主要目的是表征并验证从采矿后废料中分离出的硫化物氧化细菌（SOB）菌群通过生成元素硫从工业浸出液中去除硫化物的有效性。分离出的SOB具有由基因编码的完整硫氧化代谢系统，且以某属为主。X射线衍射分析证实，在可控的物理化学条件下，在合成培养基中培养SOB期间，收集的沉积物中存在元素硫。培养三天后的生长产量达到约2.34 g/mol，而孵育5天后约84%的硫化物转化为元素硫。在工业废水中对分离出的SOB进行验证，证实其可用于有效降低硫化物浓度（从初始的75.3 mg/L降低约100%），但对于完全处理浸出液（达到排放限值可接受），需要与其他细菌进行生物强化以确保充分降低化学需氧量（COD）。
