Instituto de Investigaciones Bioquímicas de La Plata (INIBIOLP), CONICET-UNLP, CCT-La Plata, Facultad de Ciencias Médicas, La Plata, Argentina.
Cell Biochem Funct. 2011 Aug;29(6):452-8. doi: 10.1002/cbf.1772. Epub 2011 Jul 7.
Simvastatin is a competitive inhibitor of 3-hydroxymethylglutaryl coenzyme A reductase activity, whereas geraniol is a monoterpene with multiple pharmacologic effects on mevalonate metabolism. Both of them inhibit growth and proliferation of many cell lines. The present study was designed to determine the action of geraniol, in combination with simvastatin, by assessing their effects in vitro on human hepatocarcinoma cell line (Hep G2). The treatment of Hep G2 cells with concentrations of simvastatin or geraniol that did not inhibit cell proliferation (5 µmol·l⁻¹ of simvastatin and 50 µmol·l⁻¹ of geraniol) resulted in a significant inhibition of cell proliferation. We also examined the effect of simvastatin, geraniol and the combination of both on the biosynthesis of lipids from [¹⁴C]-acetate. Our results demonstrate that the combination of simvastatin and geraniol synergistically inhibited cholesterol biosynthesis and proliferation of Hep G2 cell line, contributing to a better understanding of the action of a component of essential oils targeting a complex metabolic pathway, which would improve the use of drugs or their combination in the fight against cancer and/or cardiovascular diseases.
辛伐他汀是 3-羟甲基戊二酰辅酶 A 还原酶活性的竞争性抑制剂，而香叶醇是一种具有多种对甲羟戊酸代谢的药理学作用的单萜。它们都能抑制许多细胞系的生长和增殖。本研究旨在通过评估香叶醇与辛伐他汀联合使用对人肝癌细胞系（Hep G2）的体外作用来确定香叶醇的作用。用不会抑制细胞增殖的辛伐他汀或香叶醇浓度（5µmol·l⁻¹辛伐他汀和 50µmol·l⁻¹香叶醇）处理 Hep G2 细胞，可显著抑制细胞增殖。我们还研究了辛伐他汀、香叶醇及其组合对[¹⁴C]-乙酸酯生物合成脂质的影响。我们的结果表明，辛伐他汀和香叶醇联合抑制胆固醇生物合成和 Hep G2 细胞系的增殖，有助于更好地了解靶向复杂代谢途径的精油成分的作用，这将提高药物或其组合在抗癌和/或心血管疾病中的应用。
