Sawada Shintaro, Okano Jun-Ichi, Imamoto Ryu, Yasunaka Yuki, Abe Ryo, Koda Masahiko, Murawaki Yoshikazu, Isomoto Hajime
Division of Medicine and Clinical Science, Department of Multidisciplinary Internal Medicine, School of Medicine, Tottori University Faculty of Medicine, Yonago 683-8504, Japan.
Yonago Acta Med. 2016 Mar;59(1):37-43. Epub 2016 Apr 1.
Geraniol is a plant-derived phytochemical possessing anti-cancer action. The anti-carcinogenic effect of geraniol was investigated in the diethylnitrosamine (DEN)-induced hepatocarcinogenic rat model.
Male Wistar rats were intraperitoneally injected with 300 μL of phosphate-buffered saline (PBS) (G1; n = 4) or DEN (100 mg/kg body weight) dissolved in PBS (G2; n = 8) every 2 weeks on experimental weeks 2, 4 and 6. The rats were treated with a low concentration (0.07%) of geraniol (G3; n = 9) and high concentration (0.35%) of geraniol (G4; n = 7) for 12 weeks. To evaluate the effects of geraniol on the DEN-induced hepatocarcinogenesis, we compared the relative liver weight, serum aspartate aminotransferase (AST), alanine aminotransferase (ALT) and alkaline phosphatase (ALP) levels and expression levels of proliferating cell nuclear antigen (PCNA) and glutathione S transferase-P (GST-P) by immunohistochemical analyses among each group.
Relative liver weight was significantly higher in G2 than in G1 (P < 0.01). Both serum AST and ALT levels were significantly higher in G2 than in G3 and in G4 (P < 0.05). Serum ALP levels did not show a significant difference among each group. Percentages of both PCNA- and GST-P- positive area were significantly decreased in G3 and in G4 compared to in G2 (P < 0.001, respectively), suggesting anti-hepatocarcinogenic effects of geraniol.
Geraniol is a promising compound useful for suppression of hepatocellular carcinoma. The mechanisms of action are required to be clarified in the future intensive study.
香叶醇是一种具有抗癌作用的植物源植物化学物质。在二乙基亚硝胺（DEN）诱导的肝癌大鼠模型中研究了香叶醇的抗癌作用。
在实验第2、4和6周，每2周给雄性Wistar大鼠腹腔注射300μL磷酸盐缓冲盐水（PBS）（G1组；n = 4）或溶解于PBS中的DEN（100mg/kg体重）（G2组；n = 8）。用低浓度（0.07%）的香叶醇（G3组；n = 9）和高浓度（0.35%）的香叶醇（G4组；n = 7）对大鼠进行12周的治疗。为了评估香叶醇对DEN诱导的肝癌发生的影响，我们通过免疫组织化学分析比较了各组的相对肝脏重量、血清天冬氨酸转氨酶（AST）、丙氨酸转氨酶（ALT）和碱性磷酸酶（ALP）水平以及增殖细胞核抗原（PCNA）和谷胱甘肽S转移酶-P（GST-P）的表达水平。
G2组的相对肝脏重量显著高于G1组（P < 0.01）。G2组的血清AST和ALT水平均显著高于G3组和G4组（P < 0.05）。各组血清ALP水平无显著差异。与G2组相比，G3组和G4组的PCNA和GST-P阳性面积百分比均显著降低（分别为P < 0.001），表明香叶醇具有抗肝癌作用。
香叶醇是一种有前途的用于抑制肝细胞癌的化合物。其作用机制有待未来深入研究阐明。
