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辐射松中3':5'-环磷酸腺苷的分离、表征及分布

Isolation, characterization and distribution of adenosine 3':5'-cyclic monophosphate from Pinus radiata.

作者信息

Wilson T, Moustafa E, Renwick A G

出版信息

Biochem J. 1978 Dec 1;175(3):931-6. doi: 10.1042/bj1750931.

DOI:10.1042/bj1750931
PMID:217358
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1186155/
Abstract

Cyclic AMP was extracted in 0.1 M-HCl from tissues of Pinus radiata and purified by gel filtration on Sephadex G-10, and chromatography on Dowex AG1 (X2) and polyethyleneimine-cellulose in two separate solvent systems. Presumptive cyclic AMP from 10kg batches of pine needles was characterized by countercurrent distribution in the presence of cyclic [8-3H]AMP. Statistical analysis of the curves for radioactivity and mass (determined by the Gilman competitive-binding assay) showed that the fit of the curves was highly significant for seven degrees of freedom. The distribution of cyclic AMP within P. radiata and various other plant tissues was determined by the Gilman procedure. The results suggest that there is no relationship between variations in cyclic AMP concentrations and the known function of the tissue in which it was measured.

摘要

环磷酸腺苷（Cyclic AMP）是从辐射松组织中用0.1M盐酸提取的，并通过在葡聚糖凝胶G - 10上进行凝胶过滤，以及在Dowex AG1(X2)和聚乙烯亚胺纤维素上于两种不同的溶剂系统中进行色谱法进行纯化。来自10千克批量松针的推定环磷酸腺苷通过在环状[8 - 3H]AMP存在下的逆流分配进行表征。对放射性曲线和质量曲线（通过吉尔曼竞争性结合测定法测定）的统计分析表明，对于七个自由度，曲线拟合非常显著。通过吉尔曼方法测定了环磷酸腺苷在辐射松和各种其他植物组织中的分布。结果表明，环磷酸腺苷浓度的变化与测定其浓度的组织的已知功能之间没有关系。

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引用本文的文献

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Artefactual Origins of Cyclic AMP in Higher Plant Tissues.高等植物组织中环腺苷酸的人为起源。
Plant Physiol. 1989 Oct;91(2):624-8. doi: 10.1104/pp.91.2.624.

本文引用的文献

1
Attempts to detect cyclic adenosine 3':5'-monophosphate in higher plants by three assay methods.尝试用三种测定方法检测高等植物中的环腺苷酸 3':5'-单磷酸。
Plant Physiol. 1976 Jan;57(1):29-37. doi: 10.1104/pp.57.1.29.
2
Studies on the presence of adenosine cyclic 3':5'-monophosphate in oat coleoptiles.燕麦胚芽鞘中3':5'-环磷酸腺苷存在情况的研究。
Plant Physiol. 1975 Feb;55(2):346-51. doi: 10.1104/pp.55.2.346.
3
A PROCEDURE FOR THE ANALYSIS OF COUNTERCURRENT DISTRIBUTION DATA. 3. A COMPUTER PROGRAM FOR THE STATISTICAL ANALYSIS OF RADIOCHEMICAL PURITY.逆流分布数据的分析方法。3. 放射化学纯度统计分析的计算机程序。
J Biol Chem. 1965 Apr;240:1573-8.
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Cyclic nucleotides in higher plants?高等植物中的环核苷酸？
Adv Cyclic Nucleotide Res. 1974;4(0):439-60.
5
Cyclic adenosine 3',5'-monophosphate and morphogenesis in Mucor racemosus.环状3',5'-单磷酸腺苷与总状毛霉的形态发生
J Bacteriol. 1974 Feb;117(2):432-8. doi: 10.1128/jb.117.2.432-438.1974.
6
Growth control and cyclic alterations of cyclic AMP in the cell cycle.细胞周期中的生长控制与环磷酸腺苷的周期性变化
Nat New Biol. 1972 Oct 11;239(93):161-3. doi: 10.1038/newbio239161a0.
7
A simple and sensitive saturation assay method for the measurement of adenosine 3':5'-cyclic monophosphate.一种用于测量3':5'-环磷酸腺苷的简单且灵敏的饱和测定方法。
Biochem J. 1971 Feb;121(3):561-2. doi: 10.1042/bj1210561.
8
Mass spectrometry of nucleic acid components. Trimethylsilyl derivatives of nucleotides.核酸成分的质谱分析。核苷酸的三甲基硅烷基衍生物。
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A protein binding assay for adenosine 3':5'-cyclic monophosphate.一种用于检测3':5'-环磷酸腺苷的蛋白质结合测定法。
Proc Natl Acad Sci U S A. 1970 Sep;67(1):305-12. doi: 10.1073/pnas.67.1.305.
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Cyclic adenosine monophosphate in bacteria.细菌中的环磷酸腺苷
Science. 1970 Jul 24;169(3943):339-44. doi: 10.1126/science.169.3943.339.