辐射松中3':5'-环磷酸腺苷的分离、表征及分布

Isolation, characterization and distribution of adenosine 3':5'-cyclic monophosphate from Pinus radiata.

作者信息

Wilson T, Moustafa E, Renwick A G

出版信息

Biochem J. 1978 Dec 1;175(3):931-6. doi: 10.1042/bj1750931.

DOI:10.1042/bj1750931

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1186155/

Abstract

Cyclic AMP was extracted in 0.1 M-HCl from tissues of Pinus radiata and purified by gel filtration on Sephadex G-10, and chromatography on Dowex AG1 (X2) and polyethyleneimine-cellulose in two separate solvent systems. Presumptive cyclic AMP from 10kg batches of pine needles was characterized by countercurrent distribution in the presence of cyclic [8-3H]AMP. Statistical analysis of the curves for radioactivity and mass (determined by the Gilman competitive-binding assay) showed that the fit of the curves was highly significant for seven degrees of freedom. The distribution of cyclic AMP within P. radiata and various other plant tissues was determined by the Gilman procedure. The results suggest that there is no relationship between variations in cyclic AMP concentrations and the known function of the tissue in which it was measured.

摘要

环磷酸腺苷（Cyclic AMP）是从辐射松组织中用0.1M盐酸提取的，并通过在葡聚糖凝胶G - 10上进行凝胶过滤，以及在Dowex AG1(X2)和聚乙烯亚胺纤维素上于两种不同的溶剂系统中进行色谱法进行纯化。来自10千克批量松针的推定环磷酸腺苷通过在环状[8 - 3H]AMP存在下的逆流分配进行表征。对放射性曲线和质量曲线（通过吉尔曼竞争性结合测定法测定）的统计分析表明，对于七个自由度，曲线拟合非常显著。通过吉尔曼方法测定了环磷酸腺苷在辐射松和各种其他植物组织中的分布。结果表明，环磷酸腺苷浓度的变化与测定其浓度的组织的已知功能之间没有关系。

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