Division of Applied Bioscience, Laboratory of Nutritional Biochemistry, Research Faculty of Agriculture, Hokkaido University, Kita-9, Nishi-9, Kita-ku, Sapporo 060-8589, Japan.
Br J Nutr. 2011 Oct;106(8):1190-7. doi: 10.1017/S000711451100167X. Epub 2011 Jun 2.
Short-chain fructo-oligosaccharides (FOS) are known to have beneficial effects on health. However, the effects of FOS on insulin resistance have not been fully clarified. We observed the effects of FOS feeding on insulin sensitivity and adipocytokine release from abdominal adipocytes in weaning rats. Male Sprague-Dawley rats, 3 weeks old, were divided into three groups and fed a sucrose-based American Institute of Nutrition (AIN)-93 growth diet (control), the control diet containing 5 % FOS for 5 weeks (FOS-5wk) or the control diet for 2 weeks followed by the 5 % FOS diet for 3 weeks (FOS-3wk). Tail blood was collected after fasting for 9 h on day 33 of feeding, and glucose and insulin levels were measured. On the last day, rats were anaesthetised and killed after the collection of aortic blood. Small- and large-intestinal mesenteric fat tissues were immediately excised, and the release of adiponectin, leptin and TNF-α was evaluated from the subsequently isolated adipocytes. The weight of the large-intestinal mesenteric fat, fasting blood insulin level and homeostatic model assessment for insulin resistance decreased in a time-dependent manner, and were much lower in the FOS-5wk group than in the control group. These values were correlated with aortic blood leptin levels. The secretion rate of leptin from the isolated mesenteric adipocytes in the small intestine, but not in the large intestine, was lower in the FOS-fed groups than in the control group. In conclusion, FOS feeding improved insulin sensitivity accompanied by the reduction in large-intestinal fat mass and leptin secretion from the mesenteric adipocytes of the small intestine.
短链果糖低聚糖 (FOS) 已知对健康有益。然而，FOS 对胰岛素抵抗的影响尚未完全阐明。我们观察了 FOS 喂养对断奶大鼠胰岛素敏感性和内脏脂肪细胞 adipocytokine 释放的影响。3 周龄雄性 Sprague-Dawley 大鼠分为 3 组，分别喂食蔗糖型美国营养学会 (AIN)-93 生长饮食 (对照组)、含 5% FOS 的对照饮食 5 周 (FOS-5wk) 或对照饮食 2 周后再喂食 3 周 5% FOS 饮食 (FOS-3wk)。喂养第 33 天禁食 9 小时后采集尾血，测量血糖和胰岛素水平。最后一天，麻醉大鼠，采集主动脉血后处死。立即切除小、大肠肠系膜脂肪组织，从随后分离的脂肪细胞中评估 adiponectin、leptin 和 TNF-α 的释放。大肠肠系膜脂肪重量、空腹胰岛素水平和胰岛素抵抗的稳态模型评估呈时间依赖性降低，FOS-5wk 组明显低于对照组。这些值与主动脉血 leptin 水平相关。FOS 喂养组从小肠肠系膜脂肪细胞中分离出的 leptin 分泌率低于对照组，但从大肠肠系膜脂肪细胞中分离出的 leptin 分泌率无差异。总之，FOS 喂养可改善胰岛素敏感性，同时减少大肠脂肪量和小肠肠系膜脂肪细胞 leptin 的分泌。
