慢性乙型肝炎患者可溶性共刺激分子 CTLA-4 和 CD28 的异常产生。
Aberrant production of soluble co-stimulatory molecules CTLA-4 and CD28 in patients with chronic hepatitis B.
机构信息
Department of Laboratory Medicine, The First Affiliated Hospital of Chongqing Medical University, No. 1 Youyi Road, Yuzhong District, Chongqing 400016, China.
出版信息
Microb Pathog. 2011 Oct;51(4):262-7. doi: 10.1016/j.micpath.2011.06.003. Epub 2011 Jun 29.
Co-stimulation signals are critical for anti-viral immunity. Here we investigated whether there is altered expression and the clinical significance of soluble co-stimulatory molecules in patients with chronic HBV infection. Serum concentrations and ex vivo production of soluble CTLA-4, CD28, CD80 and CD86 were analyzed by enzyme-linked immunosorbent assay. Serum sCTLA-4 and sCD28 concentrations in all chronic HBV patients were significantly higher than concentrations in healthy control subjects. Serum sCTLA-4 and sCD28 correlated significantly with alanine aminotransferase in all chronic HBV patients. Upon mitogen stimulation of peripheral blood mononuclear cells, the percentage increases in ex vivo production of sCD28 and sCD80 and the percentage decrease in sCTLA-4 release were all significantly lower in chronic HBV patients than those in healthy subjects. The aberrant production of soluble co-stimulatory molecules should reflect the dysregulation of T cell activation and is related to the pathogenesis and severity of chronic HBV infection.
共刺激信号对于抗病毒免疫至关重要。在这里，我们研究了慢性乙型肝炎病毒感染患者中可溶性共刺激分子的表达改变及其临床意义。通过酶联免疫吸附试验分析了血清中可溶性 CTLA-4、CD28、CD80 和 CD86 的浓度和体外产生情况。所有慢性乙型肝炎病毒患者的血清 sCTLA-4 和 sCD28 浓度明显高于健康对照组。所有慢性乙型肝炎病毒患者的血清 sCTLA-4 和 sCD28 与丙氨酸氨基转移酶显著相关。在有丝分裂原刺激外周血单个核细胞后，慢性乙型肝炎病毒患者体外产生 sCD28 和 sCD80 的百分比增加以及 sCTLA-4 释放的百分比降低均明显低于健康对照组。可溶性共刺激分子的异常产生反映了 T 细胞激活的失调，与慢性乙型肝炎病毒感染的发病机制和严重程度有关。
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