Departamento de Química Analítica, Universidad de Valencia, C. Doctor Moliner 50, E-46100 Burjassot, Valencia, Spain.
J Chromatogr A. 2011 Oct 21;1218(42):7528-33. doi: 10.1016/j.chroma.2011.06.075. Epub 2011 Jun 26.
A simple and reliable method for the evaluation of triacylglycerols (TAGs) in vegetable oils by capillary electrochromatography (CEC) with UV-Vis detection, using octadecyl acrylate (ODA) ester-based monolithic columns, has been developed. The percentages of the porogenic solvents in the polymerization mixture, and the mobile phase composition, were optimized. The optimum monolith was obtained at the following ratios: 40:60% (wt/wt) monomers/porogens, 60:40% (wt/wt) ODA/1,3-butanediol diacrylate and 23:77% (wt/wt) 1,4-butanediol/1-propanol (14 wt% 1,4-butanediol in the polymerization mixture). A satisfactory resolution between TAGs was achieved in less than 12 min with a 65:35 (v/v) acetonitrile/2-propanol mixture containing 5 mM ammonium acetate. The method was applied to the analysis of TAGs of vegetable oil samples. Using linear discriminant analysis of the CEC TAG profiles, the vegetable oils belonging to six different botanical origins (corn, extra virgin olive, hazelnut, peanut, soybean and sunflower) were correctly classified with an excellent resolution among all the categories.
已经开发出一种通过毛细管电色谱（CEC）与紫外可见检测相结合，使用十八烷基丙烯酰胺（ODA）酯基整体柱来评估植物油中三酰基甘油（TAG）的简单可靠的方法。优化了聚合混合物中致孔剂溶剂的百分比和流动相组成。在以下比例下获得了最佳整体柱：单体/致孔剂为 40:60%（wt/wt），ODA/1,3-丁二醇二丙烯酸酯为 60:40%（wt/wt），1,4-丁二醇/1-丙醇为 23:77%（wt/wt）（聚合混合物中的 14wt%1,4-丁二醇）。在不到 12 分钟的时间内，使用包含 5 mM 乙酸铵的 65:35（v/v）乙腈/2-丙醇混合物即可实现 TAG 之间令人满意的分离。该方法已应用于植物油样品中 TAG 的分析。通过对 CEC TAG 图谱的线性判别分析，正确分类了属于六个不同植物来源（玉米、特级初榨橄榄油、榛子、花生、大豆和向日葵）的植物油，所有类别之间的分辨率均非常好。
