一种确定配体对DNA中A-T和G-C碱基对相对影响的方法：应用于金属离子、质子和两种氨基酸。
A method for determining the relative effect of ligands on A-T and G-C base pairs in DNA: applications to metal ions, protons and two amino acids.
作者信息
Haroutiunian S G, Dalian E B, Aslanian V M, Akhrem A A
机构信息
Department of Molecular Physics and Biophysics, Yerevan State University, USSR.
出版信息
Nucleic Acids Res. 1990 Nov 11;18(21):6413-7. doi: 10.1093/nar/18.21.6413.
A new method is described for the study of specific interactions of low-molecular ligands with the base pairs of DNA. This method is based on the comparative analysis of melting temperature changes in DNAs of different GC-content in the presence of low molecular weight ligands. In this paper, the method is applied to Mn2+, Ni2+, Co2+ ions, deprotonation, amino acids, beta-alanine and gamma-aminobutyric acid (gamma-ABA). Differences in Tm are affected not only by the changes of relative stability of AT- and GC-pairs, but also by other factors. A theoretical analysis of the sequence specificity of low-molecular ligands on the base pairs in DNA molecules characterized by a high degree of sequence heterogeneity is also presented.
本文描述了一种研究低分子配体与DNA碱基对特异性相互作用的新方法。该方法基于在低分子量配体存在下，对不同GC含量的DNA解链温度变化进行比较分析。本文将该方法应用于Mn2+、Ni2+、Co2+离子、去质子化作用、氨基酸、β-丙氨酸和γ-氨基丁酸（γ-ABA）。解链温度的差异不仅受AT对和GC对相对稳定性变化的影响，还受其他因素影响。本文还对具有高度序列异质性的DNA分子中低分子配体与碱基对的序列特异性进行了理论分析。
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