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1
Effect of guanine nucleotides on polyphosphoinositide synthesis in rat liver plasma membranes.鸟嘌呤核苷酸对大鼠肝细胞膜中多磷酸肌醇合成的影响。
Biochem J. 1990 Nov 1;271(3):591-7. doi: 10.1042/bj2710591.
2
Guanine-nucleotide and hormone regulation of polyphosphoinositide phospholipase C activity of rat liver plasma membranes. Bivalent-cation and phospholipid requirements.鸟嘌呤核苷酸和激素对大鼠肝细胞膜多磷酸肌醇磷脂酶C活性的调节。二价阳离子和磷脂需求
Biochem J. 1987 Dec 15;248(3):791-9. doi: 10.1042/bj2480791.
3
Regulation of brain phosphatidylinositol-4-phosphate kinase by GTP analogues. A potential role for guanine nucleotide regulatory proteins.GTP类似物对脑磷脂酰肌醇-4-磷酸激酶的调节。鸟嘌呤核苷酸调节蛋白的潜在作用。
J Biol Chem. 1989 Feb 25;264(6):3206-10.
4
Phosphoinositide hydrolysis by guanosine 5'-[gamma-thio]triphosphate-activated phospholipase C of turkey erythrocyte membranes.火鸡红细胞膜中由鸟苷5'-[γ-硫代]三磷酸激活的磷脂酶C引起的磷酸肌醇水解作用。
Biochem J. 1988 Jun 1;252(2):583-93. doi: 10.1042/bj2520583.
5
Hormone-stimulated polyphosphoinositide breakdown in rat liver plasma membranes. Roles of guanine nucleotides and calcium.激素刺激大鼠肝细胞膜中多磷酸肌醇的分解。鸟嘌呤核苷酸和钙的作用。
J Biol Chem. 1986 Feb 15;261(5):2140-6.
6
The roles of phospholipase D and a GTP-binding protein in guanosine 5'-[gamma-thio]triphosphate-stimulated hydrolysis of phosphatidylcholine in rat liver plasma membranes.磷脂酶D和一种GTP结合蛋白在鸟苷5'-[γ-硫代]三磷酸刺激大鼠肝细胞膜中磷脂酰胆碱水解中的作用。
Biochem J. 1990 Dec 15;272(3):749-53. doi: 10.1042/bj2720749.
7
Reconstitution of thromboxane A2 receptor-stimulated phosphoinositide hydrolysis in isolated platelet membranes: involvement of phosphoinositide-specific phospholipase C-beta and GTP-binding protein Gq.在分离的血小板膜中血栓素A2受体刺激的磷酸肌醇水解的重建：磷酸肌醇特异性磷脂酶C-β和GTP结合蛋白Gq的参与
Biochem J. 1993 Apr 1;291 ( Pt 1)(Pt 1):235-40. doi: 10.1042/bj2910235.
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The role of nucleoside-diphosphate kinase reactions in G protein activation of NADPH oxidase by guanine and adenine nucleotides.核苷二磷酸激酶反应在鸟嘌呤和腺嘌呤核苷酸对NADPH氧化酶的G蛋白激活中的作用。
Eur J Biochem. 1988 Jul 15;175(1):51-5. doi: 10.1111/j.1432-1033.1988.tb14165.x.
9
Dual effect of fluoride on phosphoinositide metabolism in rat brain cortex. Stimulation of phospholipase C and inhibition of polyphosphoinositide synthesis.氟对大鼠大脑皮层磷酸肌醇代谢的双重作用。刺激磷脂酶C并抑制多磷酸肌醇合成。
Biochem J. 1990 Jun 15;268(3):733-7. doi: 10.1042/bj2680733.
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Guanine nucleotides stimulate polyphosphoinositide phosphodiesterase and exocytotic secretion from HL60 cells permeabilized with streptolysin O.鸟嘌呤核苷酸刺激用链球菌溶血素O通透处理的HL60细胞中的多磷酸肌醇磷酸二酯酶和胞吐分泌。
Biochem J. 1988 Mar 1;250(2):375-82. doi: 10.1042/bj2500375.

引用本文的文献

1
Activation of phosphatidylinositol 4,5-bisphosphate supply by agonists and non-hydrolysable GTP analogues.激动剂和不可水解的GTP类似物对磷脂酰肌醇4,5-二磷酸供应的激活作用。
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本文引用的文献

1
Vasopressin-, angiotensin II-, and alpha 1-adrenergic-induced inhibition of Ca2+ transport by rat liver plasma membrane vesicles.血管加压素、血管紧张素II和α1-肾上腺素能诱导大鼠肝细胞膜囊泡对Ca2+转运的抑制作用。
J Biol Chem. 1984 Feb 10;259(3):1382-5.
2
Quantitation and early kinetics of inositol lipid changes induced by vasopressin in isolated and cultured hepatocytes.血管加压素诱导的离体培养肝细胞中肌醇脂质变化的定量分析及早期动力学
J Biol Chem. 1983 May 10;258(9):5716-25.
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12-O-Tetradecanoylphorbol 13-acetate stimulates inositol lipid phosphorylation in intact human platelets.
FEBS Lett. 1984 Aug 6;173(2):389-93. doi: 10.1016/0014-5793(84)80811-x.
4
Inositol trisphosphate and diacylglycerol as second messengers.肌醇三磷酸和二酰甘油作为第二信使。
Biochem J. 1984 Jun 1;220(2):345-60. doi: 10.1042/bj2200345.
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Kinase A does not activate phosphatidylinositol phosphorylation in rat lymphocyte membranes.
Am J Physiol. 1986 Dec;251(6 Pt 1):C883-6. doi: 10.1152/ajpcell.1986.251.6.C883.
6
Ethanol does not stimulate guanine nucleotide-induced activation of phospholipase C in permeabilized hepatocytes.乙醇不会刺激鸟嘌呤核苷酸诱导的通透化肝细胞中磷脂酶C的激活。
Arch Biochem Biophys. 1987 Jul;256(1):29-38. doi: 10.1016/0003-9861(87)90422-x.
7
Inhibition of agonist-induced platelet aggregation, Ca2+ mobilization and granule secretion by guanosine 5'-[beta-thio]diphosphate and GDP in intact platelets. Evidence for an inhibitory mechanism unrelated to the inhibition of G-protein-GTP interaction.鸟苷5'-[β-硫代]二磷酸和GDP对完整血小板中激动剂诱导的血小板聚集、Ca2+动员及颗粒分泌的抑制作用。一种与抑制G蛋白-GTP相互作用无关的抑制机制的证据。
Biochem J. 1988 Feb 15;250(1):209-14. doi: 10.1042/bj2500209.
8
Phosphatidate accumulation in hormone-treated hepatocytes via a phospholipase D mechanism.通过磷脂酶D机制在激素处理的肝细胞中积累磷脂酸。
J Biol Chem. 1987 Nov 5;262(31):15309-15.
9
Stimulation of phospholipase A2 activity in bovine rod outer segments by the beta gamma subunits of transducin and its inhibition by the alpha subunit.转导蛋白的βγ亚基对牛视杆细胞外段磷脂酶A2活性的刺激作用及其α亚基对该活性的抑制作用。
Proc Natl Acad Sci U S A. 1987 Jun;84(11):3623-7. doi: 10.1073/pnas.84.11.3623.
10
Polyphosphoinositide labeling in rat liver plasma membranes is reduced by preincubation with cholera toxin.
J Biol Chem. 1987 Jun 5;262(16):7744-50.

鸟嘌呤核苷酸对大鼠肝细胞膜中多磷酸肌醇合成的影响。

Effect of guanine nucleotides on polyphosphoinositide synthesis in rat liver plasma membranes.

作者信息

Benistant C, Thomas A P, Rubin R

机构信息

Department of Pathology and Cell Biology, Jefferson Medical College, Philadelphia, PA 19107.

出版信息

Biochem J. 1990 Nov 1;271(3):591-7. doi: 10.1042/bj2710591.

DOI:10.1042/bj2710591
PMID:2173901
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1149603/
Abstract

The effect of guanosine 5'-[gamma-thio]triphosphate (GTP[S]) on PtdIns and PtdIns(4)P kinase activities was measured in rat liver plasma membranes. The addition of [32P]ATP resulted in the rapid incorporation of 32P into PtdIns(4)P and PtdIns(4,5)P2, with maximal levels reached within 30 s. GTP[S] (25-500 microM) increased the rate and magnitude of [32P]PtdIns(4)P and [32P]PtdIns(4,5)P2 formation by 50 and 120% respectively. Similar stimulatory effects were induced by guanosine 5'-[beta gamma-imido]triphosphate, GTP, GDP and guanosine 5'-[beta-thio]diphosphate. The stimulation of PtdIns phosphorylation by GTP[S] occurred in the presence of 2 mM-EGTA, a condition which fully inhibited phosphoinositide-specific phospholipase C. GTP[S] did not stimulate phosphomonoesterase activity, and its action was not due to the binding of magnesium. However, the overall ATP-hydrolysing activity of the membrane preparation was inhibited by GTP[S] and the other guanine nucleotides. There was a direct correlation between the extent of this inhibition and the stimulation of polyphosphoinositide formation. The results indicate that stimulation of polyphosphoinositide formation by guanine nucleotides in rat liver plasma membranes can be accounted for by an inhibition of ATP hydrolysis. These data are inconsistent with a specific GTP-binding protein (G-protein)-mediated stimulation of PtdIns or PtdIns(4)P kinase.

摘要

在大鼠肝细胞膜中测定了鸟苷5'-[γ-硫代]三磷酸（GTP[S]）对磷脂酰肌醇（PtdIns）和磷脂酰肌醇-4-磷酸（PtdIns(4)P）激酶活性的影响。加入[32P]ATP后，32P迅速掺入PtdIns(4)P和磷脂酰肌醇-4,5-二磷酸（PtdIns(4,5)P2）中，30秒内达到最高水平。GTP[S]（25 - 500微摩尔）使[32P]PtdIns(4)P和[32P]PtdIns(4,5)P2的形成速率和量分别增加了50%和120%。鸟苷5'-[βγ-亚氨基]三磷酸、GTP、GDP和鸟苷5'-[β-硫代]二磷酸也诱导了类似的刺激作用。在存在2毫摩尔乙二醇双四乙酸（EGTA）的情况下，GTP[S]刺激了PtdIns磷酸化，而这种条件完全抑制了磷酸肌醇特异性磷脂酶C。GTP[S]不刺激磷酸单酯酶活性，其作用也不是由于镁的结合。然而，膜制剂的总体ATP水解活性受到GTP[S]和其他鸟嘌呤核苷酸的抑制。这种抑制程度与多磷酸肌醇形成的刺激之间存在直接相关性。结果表明，鸟嘌呤核苷酸对大鼠肝细胞膜中多磷酸肌醇形成的刺激作用可归因于ATP水解的抑制。这些数据与特定的GTP结合蛋白（G蛋白）介导的PtdIns或PtdIns(4)P激酶刺激作用不一致。