评估 RbCl 和 CrCl₃ 作为巴西红蝽（半翅目：红蝽科）若虫的标记物：Rb 和 Cr 对三锥虫生物学的持久性和影响。
Evaluation of RbCl and CrCl₃ as markers of Triatoma brasiliensis (Hemiptera: Reduviidae) nymphs: persistence and influence of Rb and Cr on triatomine biology.
机构信息
Laboratório de Transmissores de Hematozoários, Instituto Oswaldo Cruz-Fiocruz, Rio de Janeiro, RJ, Brasil.
出版信息
Mem Inst Oswaldo Cruz. 2011 Jun;106(4):385-9. doi: 10.1590/s0074-02762011000400001.
In order to mark Triatoma brasiliensis, the vector of Chagas disease in Brazil, two chemical compounds, rubidium chloride (RbCl) and chromium chloride (CrCl₃), were tested. First, 199 N2-N5 nymphs were fed on blood with 0.025M RbCl. Rb marker positivity ranged from 2.5% (N3)-70% (N2), with a maximum persistence of 98 days. Second, 265 N2-N5 nymphs were fed on blood containing 0.0015M CrCl₃. Cr marker positivity ranged up to 93% (N5), with a maximum persistence of 119 days. Finally, we blood fed 213 T. brasiliensis to investigate whether CrCl₃ altered the biology of this insect. The developmental time of T. brasiliensis was unaltered, but the survival of the Cr-marked group was lower than that of the control group. Differences in the mean fecundity of the control (mean of 156.1) and experimental (mean of 135.6) groups were not statistically significant and 100% of the egg batches of females Cr-marked as nymphs were positive. In conclusion, CrCl₃ is a useful tool for marking T. brasiliensis nymphs due to its high positivity and persistence.
为了标记巴西恰加斯病的传播媒介——三锥齿蝽（Triatoma brasiliensis），我们测试了两种化学化合物，氯化铷（RbCl）和氯化铬（CrCl₃）。首先，我们用含有 0.025M RbCl 的血液喂养了 199 只 N2-N5 龄若虫。Rb 标记阳性率从 2.5%（N3）到 70%（N2）不等，最大持续时间为 98 天。其次，我们用含有 0.0015M CrCl₃ 的血液喂养了 265 只 N2-N5 龄若虫。Cr 标记阳性率高达 93%（N5），最大持续时间为 119 天。最后，我们用 213 只三锥齿蝽进行了血液喂养实验，以研究 CrCl₃是否会改变这种昆虫的生物学特性。三锥齿蝽的发育时间没有改变，但 Cr 标记组的存活率低于对照组。对照组（平均 156.1）和实验组（平均 135.6）的平均产卵量差异无统计学意义，且 100%的 Cr 标记雌虫卵均为阳性。综上所述，CrCl₃是一种标记三锥齿蝽若虫的有用工具，因为它具有高阳性率和持久性。
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