Dra. Inés Albesa. Dpto. Farmacia- Facultad de Ciencias Químicas, Universidad Nacional de Córdoba. Haya de la Torre y Medina Allende. Ciudad Universitaria, Córdoba, Argentina.
Cell Biochem Biophys. 2011 Dec;61(3):467-72. doi: 10.1007/s12013-011-9227-z.
Proteins and lipids maybe important targets of oxidation and this may alter their functions. We evaluated whether ceftazidima (CAZ), piperacillin (PIP), chloramphenicol (CMP), and ciprofloxacin (CIP) could oxidize the macromolecules in the three bacterial genera Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa. There was an increase in lipid peroxidation observed in these three species. However, this was lower in the Gram negative bacteria than in S. aureus. A reduction of the carbonyl residue in S. aureus with ciprofloxacin was observed whereas in Gram negative bacteria the antibiotics increased the carbonyl residue with respect to the control. Although the strains suffered a rise in advanced oxidation protein products (AOPP) in the presence of ciprofloxacin, the S. aureus strain had a smaller increase of AOPP than the other strains. The results described in this article provide data about the susceptibility of the three bacterial genera to the oxidative stress induced by the antibiotics studied.
蛋白质和脂质可能是氧化的重要靶点，这可能会改变它们的功能。我们评估了头孢他啶（CAZ）、哌拉西林（PIP）、氯霉素（CMP）和环丙沙星（CIP）是否可以氧化金黄色葡萄球菌、大肠杆菌和铜绿假单胞菌这三种细菌属中的大分子。在这三种细菌中观察到脂质过氧化增加。然而，革兰氏阴性菌的脂质过氧化程度低于金黄色葡萄球菌。与对照组相比，环丙沙星使金黄色葡萄球菌的羰基残基减少，而在革兰氏阴性菌中，抗生素使羰基残基增加。尽管在存在环丙沙星的情况下，三种细菌的菌株的晚期氧化蛋白产物（AOPP）增加，但金黄色葡萄球菌菌株的 AOPP 增加幅度小于其他菌株。本文所述的结果提供了关于三种细菌属对所研究抗生素诱导的氧化应激的敏感性的数据。
