Atmospheric Carbon Dioxide Modifies the Antimicrobial Activity and Oxidative Stress Generated by Ciprofloxacin in .

The accelerated increase in atmospheric CO concentration is one of the most pressing problems at present. It is possible that this increase causes slight modifications in intracellular CO. The aim of this work was to determine whether CO at different concentrations can affect the oxidative damage caused by ciprofloxacin (CIP) in and to evaluate the possible implications of this effect for human health. To identify the effects of CO on the action of CIP, reactive oxygen (ROS) and reactive nitrogen (RNS) species were measured at two different CO concentrations while monitoring the bacterial antioxidant response. These assays showed that CO led to a decrease in ROS formation relative to that under atmospheric conditions (ACs), while it had the opposite effect on RNS formation, which increased relative to that under ACs. Under CO conditions, antioxidant defenses were less activated, with superoxide dismutase, catalase, and ferric reducing assay potency decreasing compared to those under ACs; however, reduced glutathione exhibited the opposite behavior. In the presence of CO, the activity of CIP against was reduced relative to that under ACs. In conclusion, CO interferes with the action of CIP in bacterial cells, generating changes in oxidative stress.