Cano Aristizábal Viviana, Mendoza Ocampo Elia Soledad, Quinteros Melisa de Los Ángeles, Paraje María Gabriela, Páez Paulina Laura
Department of Pharmaceutical Sciences, Faculty of Chemical Sciences, National University of Córdoba, Córdoba X5000HUA, Argentina.
Pharmaceutical Technology Research and Development Unit (UNITEFA), National Scientific and Technical Research Council (CONICET), Córdoba X5000HUA, Argentina.
Pathogens. 2025 Jul 14;14(7):689. doi: 10.3390/pathogens14070689.
The accelerated increase in atmospheric CO concentration is one of the most pressing problems at present. It is possible that this increase causes slight modifications in intracellular CO. The aim of this work was to determine whether CO at different concentrations can affect the oxidative damage caused by ciprofloxacin (CIP) in and to evaluate the possible implications of this effect for human health. To identify the effects of CO on the action of CIP, reactive oxygen (ROS) and reactive nitrogen (RNS) species were measured at two different CO concentrations while monitoring the bacterial antioxidant response. These assays showed that CO led to a decrease in ROS formation relative to that under atmospheric conditions (ACs), while it had the opposite effect on RNS formation, which increased relative to that under ACs. Under CO conditions, antioxidant defenses were less activated, with superoxide dismutase, catalase, and ferric reducing assay potency decreasing compared to those under ACs; however, reduced glutathione exhibited the opposite behavior. In the presence of CO, the activity of CIP against was reduced relative to that under ACs. In conclusion, CO interferes with the action of CIP in bacterial cells, generating changes in oxidative stress.
大气中一氧化碳（CO）浓度的加速上升是当前最紧迫的问题之一。这种增加有可能导致细胞内CO发生轻微变化。本研究的目的是确定不同浓度的CO是否会影响环丙沙星（CIP）对[具体细菌名称未给出]造成的氧化损伤，并评估这种影响对人类健康可能产生的影响。为了确定CO对CIP作用的影响，在监测细菌抗氧化反应的同时，在两种不同的CO浓度下测量了活性氧（ROS）和活性氮（RNS）物质。这些测定表明，与大气条件（ACs）下相比，CO导致ROS形成减少，而对RNS形成则有相反的影响，相对于ACs下RNS形成增加。在CO条件下，抗氧化防御的激活程度较低，与ACs下相比，超氧化物歧化酶、过氧化氢酶和铁还原能力测定值降低；然而，还原型谷胱甘肽表现出相反的行为。在有CO存在的情况下，CIP对[具体细菌名称未给出]的活性相对于ACs下降低。总之，CO干扰了CIP在细菌细胞中的作用，导致氧化应激发生变化。
