Olajuyigbe Aderonke Ariyike, Olajuyigbe Olufunmiso Olusola, Coopoosamy Roger Murugas
Department of Biochemistry, Olabisi Onabanjo University, Ago-Iwoye, Ogun State, Nigeria.
Department of Microbiology, School of Science and Technology, Babcock University, PMB 4005, Ilisan-Remo, Ogun State, Nigeria.
J Trop Med. 2020 Apr 8;2020:4087394. doi: 10.1155/2020/4087394. eCollection 2020.
With the increased incidence of antibacterial resistance in microorganisms, combining natural products from plants with antibiotics may be considered interesting alternatives for synergy to attain multitarget effects. In this study, the antioxidant activity of the methanol extract of and its interactions with antibiotics against bacteria of clinical importance were investigated. While its phytochemicals and antioxidant activities were determined by free radical scavenging assays, the antibacterial activities of the extract and its interactions with the antibiotics were determined by macrobroth dilution and the checkerboard methods. From the results, total phenolic content was 29.67 ± 1.90 mg GAE/100 g, total flavonoid content was 8.72 ± 0.08 mg QE/100 g, and total proanthocyanidin content was 1.94 ± 0.00 mg CE/100 g of dry plant material. The inhibition concentration 50% (IC) of DPPH, BHT, and ascorbic acid was equal to 0.04 ± 0.02 mg/ml, respectively. Those of the ABTS, BHT, and ascorbic acid were equal to 0.02 ± 0.02, 0.04 ± 0.03, and 0.04 ± 0.02 mg/ml, respectively. The checkerboard assay showed that combining the extract with different antibiotics resulted in synergistic (38.75%), indifferent (30%), additive (28.75%), and antagonistic (2.5%) interactions. The interactions between the extract and antibiotics resulting in enhanced antibacterial activities could have resulted from the antioxidant activities of the extract mopping up the ROS generated by the antibiotics or the ability of both extract and antibiotics simultaneously producing reactive oxygen species with deleterious effects resulting in synergistic antibacterial effects.
随着微生物对抗菌药物耐药性的增加，将植物天然产物与抗生素联合使用可能是实现多靶点效应协同作用的有趣替代方案。在本研究中，研究了[植物名称]甲醇提取物的抗氧化活性及其与抗生素对具有临床重要性的细菌的相互作用。通过自由基清除试验测定其植物化学物质和抗氧化活性，通过常量肉汤稀释法和棋盘法测定提取物的抗菌活性及其与抗生素的相互作用。结果显示，总酚含量为29.67±1.90mg GAE/100g，总黄酮含量为8.72±0.08mg QE/100g，原花青素总量为1.94±0.00mg CE/100g干植物材料。DPPH、BHT和抗坏血酸的半数抑制浓度（IC50）分别为0.04±0.02mg/ml。ABTS、BHT和抗坏血酸的半数抑制浓度分别为0.02±0.02、0.04±0.03和0.04±0.02mg/ml。棋盘法试验表明，将提取物与不同抗生素联合使用会产生协同（38.75%）、无关（30%）、相加（28.75%）和拮抗（2.5%）相互作用。提取物与抗生素之间导致抗菌活性增强的相互作用可能是由于提取物的抗氧化活性清除了抗生素产生的活性氧，或者是提取物和抗生素同时产生具有有害作用的活性氧从而导致协同抗菌作用。
