Hildebrandt S, Weiner E S, Senecal J L, Noell G S, Earnshaw W C, Rothfield N F
Department of Medicine, University of Connecticut School of Medicine, Farmington 06032.
Clin Immunol Immunopathol. 1990 Dec;57(3):399-410. doi: 10.1016/0090-1229(90)90114-6.
Anti-topoisomerase I autoantibodies (anti-topo I, anti-Scl-70) are associated with proximal scleroderma and are of prognostic significance in patients with Raynaud's disease. To establish a highly sensitive and specific system for the detection of anti-topo I, we have investigated sera from 409 patients and controls by Ouchterlony gel diffusion, Western immunoblot on chromosome proteins, and solid-phase enzyme-linked immunosorbent assay (ELISA) with purified topoisomerase I as antigen. The ELISA was more sensitive than the gel diffusion technique and was more specific than the Western immunoblot, while the immunoblot may identify additional autoantibodies.
