Geisler C, Høier-Madsen M
J Immunol Methods. 1985 Jun 25;80(2):211-9. doi: 10.1016/0022-1759(85)90022-5.
This paper describes the development of an enzyme-linked immunosorbent assay (ELISA) for the detection and quantitation of autoantibodies against the nuclear protein Scl-70. The isolation of Scl-70 from rat livers and the conditions for the ELISA are described. Compared with the already established double diffusion in gel for detection of anti-Scl-70 antibodies this ELISA has advantages.
