An enzyme-linked immunosorbent assay for autoantibodies against the nuclear protein Scl-70.
作者信息
Geisler C, Høier-Madsen M
出版信息
J Immunol Methods. 1985 Jun 25;80(2):211-9. doi: 10.1016/0022-1759(85)90022-5.
PMID:3925022
Abstract
This paper describes the development of an enzyme-linked immunosorbent assay (ELISA) for the detection and quantitation of autoantibodies against the nuclear protein Scl-70. The isolation of Scl-70 from rat livers and the conditions for the ELISA are described. Compared with the already established double diffusion in gel for detection of anti-Scl-70 antibodies this ELISA has advantages.
摘要
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